Evolving in the highlands: the case of the Neotropical Lerma live-bearing Poeciliopsis infans (Woolman, 1894) (Cyprinodontiformes: Poeciliidae) in Central Mexico

Table 6 Analyses of molecular variance (AMOVA) of the nuclear data for select groups of Poeciliopsis infans at different hierarchical arrangements

Testing assumptions	Source of variation	% of variance	Fixation index	P- value
S7
Grouped according to recovered clades and sub-clades	Among groups	45.48	Φ_CT: 0.45	< 0.0001
	Among populations within groups	28.26	Φ_SC: 0.51	< 0.0001
	Within populations	26.26	Φ_ST: 0.73	< 0.0001
	Total	100
Biogeographic regions	Among groups	-99.28	Φ_CT:-0.99	ns
	Among populations within groups	166.42	Φ_SC:0.83	< 0.0001
	Within populations	32.86	Φ_ST:0.67	< 0.0001
	Total	100
Without grouping a priori	Among populations	69.5	Φ_ST: 0.69	< 0.0001
	Within populations	30.5
	Total	100
RHO
Grouped according to recovered clades and sub-clades	Among groups	53.21	Φ_CT: 0.53	< 0.0001
	Among populations within groups	12.42	Φ_SC: 0.26	< 0.0001
	Within populations	34.37	Φ_ST: 0.65	< 0.0001
	Total	100
Biogeographic regions	Among groups	15.86	Φ_CT: 0.15	ns
	Among populations within groups	44.92	Φ_SC: 0.53	< 0.0001
	Within populations	39.22	Φ_ST: 0.60	< 0.0001
	Total	100
Without grouping a priori	Among populations	60.64	Φ_ST: 0.60	< 0.0001
	Within populations	39.36
	Total	100