Fig. 5From: Evolution of cytokinesis-related protein localization during the emergence of multicellularity in volvocine green algaeImmunofluorescence images of DRP1 localization in Gonium pectorale. Differential interference contrast (DIC) images (a, e, i, m), immunofluorescence images labeled with an anti-TsDRP1 (b, f, j, n) and an anti-tubulin alpha (c, g, k, o), and merged immunofluorescence images of DRP1 and tubulin (d, h, l, p) are shown. In vegetative cells, DRP1 was localized to cytoplasm (a-d). DRP1 was localized to a first division plane of two-celled embryo (white arrowheads in f-h), both first and second division planes of four-celled embryo (j-l), all first, second, third division planes of eight-celled embryo (n-p). The second division plane in four-celled stage was estimated by strong immunofluorescence of the anti-tubulin alpha (phycoplast). Scale bars: 10 μmBack to article page