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Table 1 Positively selected sites in octopus haemocyanin at which at least three or more selection tests identified significant positive selection. Analysis was performed for two separate alignments, containing 113 or 126 sequences respectively, and covering in total a 396 amino acid long region of the haemocyanin’s functional units f and g. Numbering of positions refers to the published full haemocyanin sequence of Enteroctopus dofleini [UniProt: O61363] [23, 24]. Significance thresholds were: P ≤ 0.10 for SLAC, ≤ 0.10 for FEL, MEME and PRIME; Posterior Probability ≥ 0.90 for FUBAR; Bayes Factor ≥ 0.50 for EF. See Additional file 5 for detailed results

From: Erratum to: Positive selection in octopus haemocyanin indicates functional links to temperature adaptation

Residue SLACa FELa MEMEa FUBARa EFa PRIMEb TreeSAAPc
pHi pK’ PCT
2383   * *         ↓(1) ↑(2–4)   
2409 * * * * *         
2410 * * *         ↓(5–8)   
2442      * (CC)      ↓(10) ↑(5,9,11)   
2469    *    CC       ↓(12,13)  
2496   * * * *      ↓(14,15)    
2503 * * * * * (P)       ↓(14,15,22–27) ↑(20)  
2545     * *       ↓(4, 20, 28–30) ↑(9,19,31,32)   
2575    *   *        ↓(33,34)  
2585   * * * * pHi CC (P) (V)     
2602   * *        ↓(35,36)    ↑(35,36)
2610   * *    pHi     ↓(37–39) ↑(40,41)    ↓(40–42) ↑(37–39)
2643    *    CC        ↓(38)
  1. Abbreviations: CC chemical composition, P polarity, V volume, pHi iso-electric point, pK’ equilibrium constant (ionization of COOH), alpha helical tendencies, PCT power to be at the C-terminal
  2. aSites at which SLAC, FEL, MEME, FUBAR or EF identified positive selection are indicated with asterisks
  3. bSites at which positive selection was inferred with PRIME. Properties under selection are indicated in bold letters and properties being conserved are indicated in parenthesis
  4. cSites at which positive selection was inferred with TreeSAAP. Magnitude of amino acid change was 8 for pHi and pK´ and 6 for pα and PCT. Arrows indicate an increase (↓) or decrease (↑) of the amino acid property. Parenthesis mark the affected branches shown in Figure 3