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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: Serotonin-immunoreactivity in the ventral nerve cord of Pycnogonida – support for individually identifiable neurons as ancestral feature of the arthropod nervous system

Fig. 4

General SLI pattern in pycnogonid walking leg ganglia. a-c: Extended horizontal sections through walking leg ganglion 2 of the investigated species, showing sub-sets of the identified SL-ir neurons. Imaris Surpass mode with applied clipping planes (a, c) or Imaris Section mode (b), tubulin (red) and SLI (green). Large arrowheads indicate course of ipsilateral iAMN neurites. Arrows follow the loop of contralaterally projecting cAMN neurites towards the median SL-ir domain. a: P. litorale. The intensely labeled neurites of the DMNs (only one DMN soma included in the section) pass the postero-ventral commissure dorsally and proceed anterior to it into the contralateral neuropil. b: Meridionale sp. Note the unspecific green labeling of the neural sheath, which is only observed in this species. Small arrowheads mark a longitudinal SL-ir axon passing through connectives and the walking leg ganglion. The soma of one cAMN is not fully included in shown section. Asterisks mark the slender contralateral neurite of a small DMN (soma not in section). Note the massive contralateral neurites of the large DMNs (somata not in section) passing directly anterior to the postero-ventral commissure. c: C. japonicus. The contralaterally projecting DMN neurites (one soma included in section) cross the midline as part of the postero-ventral commissure

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