Glutamine synthetase sequence evolution in the mycobacteria and their use as molecular markers for Actinobacteriaspeciation

Table 3 PCR primer sequences and priming sites

Name	Sequence (5'-3')	Product size:	Pair Tm (°C)	Genome Coordinates
glnA Up F	AGATGGACACGGTGGAGT	796 bp	55	2486860
glnA Up R	CTTTACTGTATCCGCGGC			2487605
AI FI	CACGGTCAGTAACGTCTGC	550 bp	55	2487524
AI RI	TCCACCTCGTAGAAGGAGC			2488081
AI FII	TTCGATTCGGTGAGCTTC	574 bp	57	2488029
AI RII	GCCGCTTGTAGGAGTTCA			2488602
AI FIII	ACGACGAGACGGGTTATG	294 bp	54	2488483
AI RIII	ATCAGCATGGCCGAGAAC			2488768
AI FIV	TGGTCTATAGCCAGCgcA	597 bp	56	2488633
AI RIV	GAGATGATTGCCAAGCGG			2489229

Polymerase chain reaction primers used to amplify the glnA1-locus of M. tuberculosis, including its' 5'- and 3' surrounding regions, as overlapping PCR fragments, which facilitated the assembly of the full target region for sequencing (2369 bp).

ISSN: 2730-7182