Figure 5From: Evolutionary genomic remodelling of the human 4q subtelomere (4q35.2)FRG1 and FRG2 histone acetylation and transcription in African apes and humans. A) Chromatin immunoprecipitation experiments on gorilla and chimpanzee lymphoblastoid cell lines before (GGO and PTR) and after Trichostatin A treatment (GGO-TSA and PTR-TSA). Chromatin was immunoprecipitated with a rabbit antiserum anti-acetyl histone H4, and a normal rabbit IgG as pre-immune (Upstate Biotechnology); the FRG2 and FRG1 promoter regions were amplified on Input DNA, pre-immune and H4Ac-immunoprecipitated samples using the primer pairs shown in Additional file 5. B) Quantification by real-time RT-PCR of the mRNA of the FRG2 and FRG1 genes; total RNA from untreated (control) and TSA-treated (TSA) GGO, PTR and HSA fibroblast cell lines was used as a template with primer pairs for FRG1 and FRG2 mRNAs. Each experiment was performed in triplicate (bars show SD).Back to article page