Figure 2

Conserved motifs of catalytically active rhoptry kinase subfamilies. Sequence logos of key regions in the kinase domain of the broader ePK superfamily and of predicted active ROPK subfamilies as they occur in the coccidian species examined. Letter height at each sequence position indicates greater conservation of that character in a multiple sequence alignment of a large set of ePK sequences (first row) and the annoted genomic sequences of each ROPK subfamily. Row labels indicate subfamily names, with the number of sequences in each alignment shown in parentheses. The ePK-conserved motifs shown are the glycine-rich loop in subdomain I, catalytic lysine in subdomain II, αC glutamate in subdomain III, catalytic loop in subdomain VIb, “DFG” in subdomain VII, “APE” in subdomain VIII, αF “DxW” in subdomain IX, and arginine in subdomain XI. The adjacent sequence sites surrounding each motif are included for context. Asterisks indicate above ePK motifs indicate the catalytic triad. Generated using the WebLogo [82] and ReportLab [83] libraries.