Widespread and persistent invasions of terrestrial habitats coincident with larval feeding behavior transitions during snail-killing fly evolution (Diptera: Sciomyzidae)

Table 3 Genes / primer information used in this study

Gene	Primer pair	References	Analyzed fragment size	Notes
Mitochondrial loci:
16S	LR-N-13398 / LR-J-12887	[105]	426 bp	Primer sequences identical to those of “Locust”
COI	LCO1490	[46]	658 bp	Together, both COI primer pairs encompass nearly the entire gene
	HCO-700ME	[106]
	C1-J-2183 / TL2-N-3014	[105]	813 bp
COII	TL2-J-3034 / TK-N-3785	[105]	681 bp	Amplify all of COII
Nuclear loci:
28S	D1F / D6R	[107]	1095 bp
Ef-1α	ScioEF1a-F	Designed herein	876 bp	CAYMGDGATTTCATYAARAACATGA
	ScioEF1a-R			GCRATGTGAGCGGTGTGRCAATCC

Analyzed fragment size is the number of base pairs remaining after primer sequences and regions of ambiguous alignment were removed.

ISSN: 2730-7182