Figure 4

Genomic divergences of the Revolver gene family offer chromosome tags. (A) PCR with the 3'-flanking region of a typical Revolver (Revolver-2) used as a single primer amplified four DNA fragments (2.3 kb, 2.8 kb, 3.3 kb, and 4.3 kb) from the rye genome, but produced no fragments from the wheat genome. With this primer, rye chromosomes 1R and 5R can be identified in the wheat genome. (B) Each DNA fragment derived from the chromosome addition lines of 1R, 5R, and 6R (AB646252-646254) was a nonautonomous element of Revolver, with its second intron and the downstream region, but with considerable structural changes at the 5' end. Of the 440,000 Triticeae EST clones, only two clones, which were from T. aestivum (615 bp) and H. vulgare (713 bp), showed similarity with Revolver cDNA across its entire length. Other EST clones had low homology in the 5' exon 1 regions.