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Table 2 List of primers used in this study

From: Enigmatic Diphyllatea eukaryotes: culturing and targeted PacBio RS amplicon sequencing reveals a higher order taxonomic diversity and global distribution

Primer name

Primer direction

Primer sequence (5′-3′)

Tm (°C)

Annealing site (5′-3′)

Reference or source

NSF83

F

GAAACTGCGAATGGCTCATT

49.7

84–103

[73]

Diphy257F

F

AAGWGGARTCATAATAACTTTTGCG

51.1

257–281

This study

Diphy453F

F

CGCAAATTACCCAATCCTG

48.9

453–471

This study

Diphy1881R

R

CGACCAAAACTCCAAAGATTTC

51.1

1860–1881

This study

1528R

R

TGATCCTTCTGCAGGTTCACCTAC

57.4

2127–2150

Adapted from [74]

SR1

R

CGGTACTTGTTCGCTATC

48

3565–3583

Ema Chao pers. comm

LR11

R

GCCAGTTATCCCTGTGGTAA

51.8

6414–6433

[75]

  1. Primer annealing site is based on Collodictyon KIVT02 sequence, start is 83 bp prior to account for NSF83s annealing site. Tm is calculated using oligocalc [52]. Primers used for Sanger sequencing are listed in Additional file 1: Table S1. The 18S rRNA gene primers Diphy257F and Diphy1881R are Diphyllatea-specific