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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: Cysteine boosters the evolutionary adaptation to CoCl2 mimicked hypoxia conditions, favouring carboplatin resistance in ovarian cancer

Fig. 4

Metabolic evolution driven by hypoxia mimicked with CoCl2 provides stronger response to carboplatin cytotoxicity. Cells response to Carboplatin exposure for 48 h of assay for a. ES2 cells with non-normalized to control values, b. ES2 cells in which values were normalized to the respective control, c. OVCAR3 cells with non-normalized to control values and d. OVCAR3 cells in which values were normalized to the respective control. N selected – cells selected under normoxia; H selected – cells selected under hypoxia; N – Normoxia; NC – Normoxia supplemented with cysteine; H – Hypoxia mimicked with CoCl2; HC – Hypoxia mimicked with CoCl2 supplemented with cysteine. In a. and c. asterisks represent statistical significance compared to the respective control (cells cultured in the same experimental condition but in a free-drug environment) within each cell line. In b. and d. Asterisks represent statistical significance compared to ancestral cells. Cardinals (#) represent statistical significance compared to N-selected cells. Data were normalized to the respective control. Results are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 or #p < 0.05, ##p < 0.01, ###p < 0.001 (A. and C. Independent samples T test and B. and D. One-way ANOVA with post hoc Tukey tests)

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