Fig. 4From: Cysteine boosters the evolutionary adaptation to CoCl2 mimicked hypoxia conditions, favouring carboplatin resistance in ovarian cancerMetabolic evolution driven by hypoxia mimicked with CoCl2 provides stronger response to carboplatin cytotoxicity. Cells response to Carboplatin exposure for 48 h of assay for a. ES2 cells with non-normalized to control values, b. ES2 cells in which values were normalized to the respective control, c. OVCAR3 cells with non-normalized to control values and d. OVCAR3 cells in which values were normalized to the respective control. N selected – cells selected under normoxia; H selected – cells selected under hypoxia; N – Normoxia; NC – Normoxia supplemented with cysteine; H – Hypoxia mimicked with CoCl2; HC – Hypoxia mimicked with CoCl2 supplemented with cysteine. In a. and c. asterisks represent statistical significance compared to the respective control (cells cultured in the same experimental condition but in a free-drug environment) within each cell line. In b. and d. Asterisks represent statistical significance compared to ancestral cells. Cardinals (#) represent statistical significance compared to N-selected cells. Data were normalized to the respective control. Results are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 or #p < 0.05, ##p < 0.01, ###p < 0.001 (A. and C. Independent samples T test and B. and D. One-way ANOVA with post hoc Tukey tests)Back to article page