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Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: Gene expression polymorphism underpins evasion of host immunity in an asexual lineage of the Irish potato famine pathogen

Fig. 3

Identification of Avrvnt1 as the cognate effector for Rpi-vnt1. a Agroinfiltration of Avrvnt1 leads to cell death in Solanum venturii 283–1 and 741–1 carrying Rpi-vnt1.1 and Rpi-vnt1.2, respectively, but not in susceptible potato cultivar Désirée; (1): Avrvnt1, (2): Avrvnt1 and Rpi-vnt1.1, (3): Rpi-vnt1.1, (4): empty vector pK7WG2 was used as a negative control in this experiment, (5): R3a and Avr3a were used as a positive control R/Avr pair in this experiment. Note that co-expression of Avrvnt1 and Rpi-vnt1.1 in potato cultivar Désirée (2, leaf on right side) also results in hypersensitive cell death. b Differential virulence of P13626 and P13527 on plants expressing Rpi-vnt1.1. c RT-PCR experiments performed with cDNA samples from infected potato leaves. Samples were collected at 3 days post-infection (dpi) with P13626 and P13527. Primers specific for the RXLR effector gene PITG_16294 were used for amplification. Amplification of the EF1a (Elongation Factor 1α) cDNA was included as positive control

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