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Table 1 Chromosomal location, length and primers used for each of the 4 genes studied

From: Patterns of geographic variation of thermal adapted candidate genes in Drosophila subobscura sex chromosome arrangements

Genea

Alignmentb

Primers (Forward; Reverse)

5’UTR

Exons

Introns

PhKgamma (CG1830, GA14880)

831 bp

(895 bp)

5′ - CATGACCTGGCGCAGTATTG - 3′

5′ - TAACAGCGGAGCGAGCAGTC - 3’

1–240

241–332

333–895

Ubc-E2H (CG2257, GA15327)

1085 bp

(1100 bp)

5’ - TCACTGTAGTCGGACATGCT - 3′

5′ - AGGAGAGCAACGTCACAGAT - 3’

1–662

663–1100

–

Hfw (CG3095, GA15922)

1073 bp

(1079 bp)

5’ - GCATTCAAGCGGTCCGTTAA - 3′

5′ - TATGTTGAGGCACTTGAGCG - 3’

 

349–1079

1–348

Nipsnap (CG9212, GA21617)

1095 bp

(1168 bp)

5’ - CATGCGACTGTGAGCCTCTT - 3′

5′ -CGCAGCAATACAACAAGTGG - 3’

1–411

412–470; 1149–1168

471–1148

  1. aGene symbol of the homologous gene in D. melanogaster and D. pseudoobscura respectively in parentheses
  2. balignment length with D.pseudoobcura in parentheses. 5’ UTR, introns and exon positions are referred relative to the D. pseudoobscura alignment