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Fig. 7 | BMC Evolutionary Biology

Fig. 7

From: Comparison of ventral organ development across Pycnogonida (Arthropoda, Chelicerata) provides evidence for a plesiomorphic mode of late neurogenesis in sea spiders and myriapods

Fig. 7

Cell proliferation in the VNC of C. brevirostris and S. cheilorhynchus instars. Labeling of acetylated tubulin (white) with nuclear counterstain (blue). C. brevirostris: 4 h EdU (red, ac). S. cheilorhynchus: 12 h BrdU + 12 h sea water (red, df’). Maximum projections (a, d) and optical sections (b, c, e–f’) of wholemount VNCs. Stippled ovals highlight internal VOs of one body half. Stars indicate cavities around which tubulin-rich cell processes converge. Black arrows highlight larger nuclei in the VO of wlg4. White arrows indicate cell bands and condensed fibrous strands extending dorsally from the VOs towards the neuropil. Asterisks mark transient posterior ganglion anlagen. a: VNC of last postlarval instar, ventral view, EdU signal shown separately on the right. Note conspicuously higher number of EdU+ cells in wlg4. b: Wlg2–4, sagittal section. Black arrowheads mark potential pycnotic bodies indicative of cell death occurring in addition to cell proliferation. White arrowheads mark the ventral longitudinal tract c: Wlg4, cross section. d: VNC of first juvenile instar, ventral view, BrdU labeling shown separately on the right. White arrowheads mark single BrdU-positive cells in the periphery of the ganglia. e & e’: Wlg3–4 of first juvenile instar, horizontal and sagittal sections. Black arrowhead indicates a PH3-labeled (yellow) mitotic cell. White arrowheads mark ventral longitudinal tracts. f & f’: Wlg3–4 of second juvenile instar, horizontal and sagittal sections. White arrowheads mark ventral longitudinal tracts. Abbreviations: ovn – ovigeral neuromere, pan – palpal neuromere, seg – subesophageal ganglion, wlg – walking leg ganglion, wln – walking leg neuromere

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