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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: Comparison of ventral organ development across Pycnogonida (Arthropoda, Chelicerata) provides evidence for a plesiomorphic mode of late neurogenesis in sea spiders and myriapods

Fig. 4

BrdU-EdU double-labeling in the VNC of a juvenile instar of Meridionale sp. Labeling of acetylated tubulin (white), BrdU (green) and EdU (red) with Hoechst nuclear counterstain (blue) after 5-day experiment (4 h BrdU exposure, 92 h sea water, 4 h EdU exposure, 20 h sea water, sacrifice). Sagittal vibratome section, anterior to the left. Arrows highlight the anterior and posterior migratory streams that penetrate through the neural sheath into the soma cortex. Arrowheads mark the ventral longitudinal tract. Stars indicate the small central cavity around which tubulin-rich cell processes converge. a Walking leg ganglia 3 and 4. The black rectangle indicates the region of interest shown in (be). The ellipse highlights the external VO-cluster of walking leg ganglion 3, featuring numerous BrdU-positive cells. be Detail of the external VO-cluster and the migratory streams penetrating into the cortex of walking leg ganglion 4. The images depict different marker combinations: b all four markers, c tubulin, BrdU and EdU, d BrdU and EdU, e EdU. Note the presence of exclusively BrdU-positive cells in deeper cortex layers (stippled areas). EdU-positive cells co-label for BrdU and are restricted to the external VO-cluster and the proximal part of the streams. Abbreviations: lm – longitudinal muscle bundles, mg – midgut, wlg – walking leg ganglion

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