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Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: Comparison of ventral organ development across Pycnogonida (Arthropoda, Chelicerata) provides evidence for a plesiomorphic mode of late neurogenesis in sea spiders and myriapods

Fig. 3

Last postlarval and first juvenile instars of Ph. femoratum and anatomy of their VNC. a Stereomicroscope images of the last postlarval instar (left) and the first juvenile instar (right), ventral view, anterior to the top, 70% ethanol preservation. Note incomplete differentiation of walking leg pair 4 in the last postlarval instar. b Maximum projections of wholemount VNCs of the last postlarval and first juvenile instars in ventral view, anterior to the top. Labeling of acetylated tubulin (white), phalloidin-F-actin (glow) and nuclear counterstain (blue). The decreasing size of the walking leg ganglia along an a-p developmental gradient is especially evident in the postlarval instar. Asterisks mark transient posterior ganglion anlagen. Abbreviations: seg – subesophageal ganglion, wl – walking leg, wlg – walking leg ganglion, wln – walking leg neuromere

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