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Fig. 1 | BMC Evolutionary Biology

Fig. 1

From: RNA sequencing of early round goby embryos reveals that maternal experiences can shape the maternal RNA contribution in a wild vertebrate

Fig. 1

Sampling, sequencing and verification of the Neogobius melanostomus maternal RNA contribution. a, Visualization of embryonic development by actin staining. 2-cell, 8-cell, > 32-cell, and organogenesis stage are depicted. Scale bar: 1 mm. See Additional file 1: Figure S1 for a full panel of developmental stages. b, Sampling. The ascending line represents water temperature at the sampling site. Dashed lines and dots indicate sampling dates and samples, respectively. The temperature curve during five days before each sampling date is colored orange. c, Developmental stage of sequenced samples in relation to the mean water temperature during five days before oviposition. Each dot represents a sample. d, The round goby orthologs of maternally contributed genes in the zebrafish [49] are highly expressed in pre-MZT N. melanostomus embryos

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