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Segmental duplications: evolution and impact among the current Lepidoptera genomes

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Abstract

Background

Structural variation among genomes is now viewed to be as important as single nucleoid polymorphisms in influencing the phenotype and evolution of a species. Segmental duplication (SD) is defined as segments of DNA with homologous sequence.

Results

Here, we performed a systematic analysis of segmental duplications (SDs) among five lepidopteran reference genomes (Plutella xylostella, Danaus plexippus, Bombyx mori, Manduca sexta and Heliconius melpomene) to understand their potential impact on the evolution of these species. We find that the SDs content differed substantially among species, ranging from 1.2% of the genome in B. mori to 15.2% in H. melpomene. Most SDs formed very high identity (similarity higher than 90%) blocks but had very few large blocks. Comparative analysis showed that most of the SDs arose after the divergence of each linage and we found that P. xylostella and H. melpomene showed more duplications than other species, suggesting they might be able to tolerate extensive levels of variation in their genomes. Conserved ancestral and species specific SD events were assessed, revealing multiple examples of the gain, loss or maintenance of SDs over time. SDs content analysis showed that most of the genes embedded in SDs regions belonged to species-specific SDs (“Unique” SDs). Functional analysis of these genes suggested their potential roles in the lineage-specific evolution. SDs and flanking regions often contained transposable elements (TEs) and this association suggested some involvement in SDs formation. Further studies on comparison of gene expression level between SDs and non-SDs showed that the expression level of genes embedded in SDs was significantly lower, suggesting that structure changes in the genomes are involved in gene expression differences in species.

Conclusions

The results showed that most of the SDs were “unique SDs”, which originated after species formation. Functional analysis suggested that SDs might play different roles in different species. Our results provide a valuable resource beyond the genetic mutation to explore the genome structure for future Lepidoptera research.

Background

Segmental duplications (SDs) are DNA fragments with near-identical sequences that are greater than 1Kb [1]. They have been recognized as important mediators of gene and genome evolution, and are considered the origins for gene gain, functional diversification, and gene family expansion [1, 2]. The outcomes of a gene duplication event may lie on lineage-specific selection. In this situation, the new gene copy has the opportunity to acquire novel or modified functions or become non-functional [3, 4]. These new copies are often important for the adaption of the species to certain environments [2]. SDs can lead to various types of genome rearrangements [5] and other genome structural changes between and within species [6,7,8].

Characterization and annotation of SDs are important for understanding the structure and evolution of a genome and have been explored in many organisms’ whole genomes [9,10,11,12,13,14,15]. Few systematically comparative analyses of SDs however have been performed until now. The most important example is primate genomes, used to understand the pattern and rates of SDs during hominid evolution [6]. Here, we performed the comparative analysis of SDs in the whole genomes of five Lepidoptera insects, diamondback moth (Plutella xylostella), Monarch butterfly (Danaus plexippus), silkworm (Bombyx mori), Carolina sphinx moth (Manduca sexta), and postman butterfly (Heliconius melpomene), to understand the roles of SDs during the evolution of Lepidoptera. Our analysis revealed that duplication activities varied in terms of number of base pairs or events among these different species. The marked difference of transposable elements (TEs) content in the flanking regions of SDs among these species of Lepidoptera suggested various formation mechanisms of SDs. Our functional analysis of the SDs indicated that gene families embedded in the SDs were different among the five genomes and these gene families may be related to species-specific adaptive evolution.

Methods

Data sources

The five Lepidoptera insect species, P. xylostella, B. mori, D. plexippus, M. sexta, and H. melpomene, were used to construct the SD map. The genome and predicted transcripts of diamondback moth was downloaded from DBM database (http://www.insect-genome.com/) [16]. The other genomes resources of Lepidoptera insects were downloaded from SilkDB (http://silkworm.genomics.org.cn/) [17], Heliconius Genome Project (http://www.butterflygenome.org/) [18], MonarchBase (http://www.insect-genome.com/) [19] and Carolina sphinx dataset (ftp://ftp.bioinformatics.ksu.edu/pub/Manduca/OGS2/).

Computational analysis of lepidoptera segmental duplications

We used the Whole-Genome Assembly Comparison (WGAC) method to detect the segmental duplications in the five Lepidoptera species. The insect genomes were first masked at 15% divergence level from transposable elements (TEs), high-copy repeats or simple sequence repeats (SSR) using RepeatMasker (Smit and Green http://www.repeatmasker.org/, version 4.0.6). We then used silkworm TE dataset [20] as repeat database to re-run the RepeatMasker to mask as much TEs as possible. All these repeats were deleted from the sequences and the remaining genome sequences were used to perform BLASTN searches against themselves with reduced affine gap extension parameters, which allowed gaps up to 1000 bp and e value (1e−20).

After discarding self-alignments, the repeat sequences were reinserted back into these alignments. These seed alignments were subsequently used as queries to search against the unmasked genome using BLASTN, which generated accurate alignment statistics. Considering the high rate of heterozygosity of these Lepidoptera species (except silkworm, which has a long history of domestication and inbreeding) [16, 18, 21], we conservatively lowered the identity threshold to 75% for alignments in order to capture more divergent SDs than under the 90% usual threshold. Selected alignments were those with a length longer than 1 kb and identity higher than 75%.

Gene content and functional annotation

Gene content of segmental duplications was accessed using the GFF files obtained from the dataset above (see data sources). We also assessed whether the molecular function, biological process, and pathway terms were over-represented in SDs using Blast2Go [22]. For each SD, we computed an expected number of genes for different biological processes based on their curated representation in the reference genome. The statistical significance of the functional GO Slim enrichment was evaluated using the Fisher’s exact test (p < 0.05).This analysis showed the GO terms that were significantly enriched among genes within SDs. Pfam was also used to annotate the function of the genes in the SDs [23].

RNA-seq analysis

We collected the RNA-seq data from published sources to access the gene expression level within and outside SDs regions. These data included different tissues or different developmental stages of diamondback moth [16], silkworm [24] and Carolina sphinx moth [25]. All the reads were mapped back to its genome using TopHat [26]. The expression abundance (RPKM) was calculated using CuffLinks [27]. The expression levels were assessed as Log10 (RPKM). Gene expression levels within and outside SDs regions as well as the variables were compared using a T-test with a Bonferroni correction.

Results and discussion

Segmental duplication maps among different Lepidoptera species

Using WGAC, we developed segmental duplication maps for each of the five Lepidoptera species’ genomes (Table 1). SD contents greatly varied among the five Lepidoptera species, ranging from 1.2% in Bombyx mori to 15.2% in Heliconius melpomene (Table 1, Additional file 1: Table S1). SDs with highest identity (≥90%) was the majority (ranging from 80% in M. sexta to 93% in D. plexippus) (Table 1). Based on our analysis, duplications varied in size from 5.6 Mbp in silkworm to 43 Mbp in P. xylostella. P. xylostella and H. melpomene showed the highest number of duplications (Table 1) suggesting that their genomes could be unstable or capable of tolerating extensive levels of variation. For example, in human, segmental duplications play an “expanding” role in genomic instability [28].

Table 1 Characterization of the SDs of the five Lepidoptera species

The analysis of the length of SDs in all five species indicated that the Lepidoptera genomes were significantly poor in large blocks (>4 Kb) (T-test, P < 0.025; Fig. 1). This is consistent with SDs data reported in Drosophila genome (Fiston-Lavier et al. 2007) and silkworm genome [29, 30]. The number of SDs in Lepidoptera decreased along with the increase in SDs length (Fig. 1a) and this was true for all five species (Fig. 1b). Eichler [31] has suggested that SDs in invertebrates are much smaller in length than in vertebrates. These differences probably reflect some evolutionary constraints imposed by the smaller size of the invertebrate genome [32].

Fig. 1
figure1

SDs length distributions among 5 Lepidoptera species. a Boxplots showing the mean and range of the numbers of SD for each SD length category for all five Lepidoptera species combined. Most SDs were found in the 1–1.5 kb length category. b Number of SDs of different length categories for each species. The size of the circle represents the proportion of SDs

We use RepeatMasker (Smit and Green http://www.repeatmasker.org/, version 4.0.6) to mask the transposable elements (TEs; masked at 15% divergence level), high-copy repeats or simple sequence repeats (SSR). Then silkworm TE dataset [20] was used as repeat database to rerun the RepeatMasker to mask as much TEs as possible. Thus, we used different repeat databases to mask the target genomes. The result showed that almost 22.6% of the silkworm genome was masked while 2.05% - 4.97% of other Lepidoptera genomes were masked (P. xylostella: 3.12%, D. plexippus: 2.05%, M. sexta: 4.97% and H. melpomene: 2.25%). Osanai-Futahashi et al. [33] have shown that TEs are enriched in the genome of silkworm and TEs may play important roles during the domestication of silkworm [34]. Thus, the high proportion of SDs in H. melpomene may result from some TEs left in the genome.

Comparative analysis of duplication maps among five Lepidoptera species

We further characterized each SD as “unique” or “shared”, depending on whether they exist in only one or multiple genomes. The comparative SD maps revealed that most of the segmental duplications were “unique” SDs (Fig. 2). For example, the number of shared SDs among the five Lepidoptera species varied from 83 in B. mori (e.g. 83 SDs from B. mori shared with other Lepidoptera genomes) to 1817 in H. melpomene (e.g. 1817 SDs from H. melpomene shared with other Lepidoptera genomes) (Fig. 3a).

Fig. 2
figure2

Duplication map comparison of five Lepidoptera species. SDs regions in each Lepidoptera species and their paralogous regions in other four genomes were shown. Different colors represent different insects. Only the best alignments were listed

Fig. 3
figure3

“Shared” and “Unique” SDs among 5 species. a “Shared” and “Unique” SDs in each genome. Red represents “Unique” SDs while black represents “Shared” SDs. b SDs classification of the five species based on the existing SDs (marked as “+”) and absence SDs (marked as “-”). We classified the SDs into five groups to analyze evolutionary history of SDs in Lepidoptera genome. Group A are the potential ancestral SDs events. Group B showed the SDs that were only lost in Noctuoidea and Bombycoidea while Groups C, D and E showed regions where duplication patterns were inconsistent with the generally accepted phylogeny. Such a scenario could arise as a result of a de novo origin of SDs or as a result of deleted events, which might have played a role in lineage-specific evolution. “n” represents the number of group A, B, C, D or E. c Identity comparison between “Shared” and “Unique” SDs in the genomes of the five Lepidopteron species. The identity of “Shared” SDs is marked as black while the identity of “Unique” SDs is marked as red

Butterflies (D. plexippus and H. melpomene) shared more SDs with each other than with the other species (Fig. 2) indicating their closer relationship. Silkworm and Carolina sphinx moth also shared more common SDs than with the other species, indicating their close relationship. These results are consistent with the phylogeny of Lepidoptera published by Regier et al. [35].

Based on the phylogeny of Lepidoptera [35], it was possible to assess the origins of some SDs within specific lineages and ancestral events of SDs. Since segments might have mutated after divergence, we attempted to map duplication events onto the phylogenetic tree using reconciliation method (software like NOTUNG). However, based on the blast search analysis, we found that the “unique” SDs could not find the homologous sequences in other Lepidoptera species. We had two speculations to explain this result: (1) The segments might have mutated after duplications or (2) SDs arose after the divergence of each lineage. In the first situation, all the copies should have mutated and evolved rapidly, resulting in sequence variation being too high to find a blast hit in other genomes. If so, it would be difficult to trace the ancestral sequences onto the phylogenetic tree using reconciliation method such as Notung. Thus, we classified the SDs into five groups (Fig. 3b) and focused on analyzing evolutionary history of SDs in Lepidoptera genome. To identify the potential ancestral SDs events, we initially focused on shared duplications among all five species (Group A) but none were identified (Fig. 3b),suggesting that the original SDs might have been lost early during the evolution of Lepidoptera or the origins of the SDs are along with the speciation of the Lepidoptera. The second situation would lead to some SDs that only exist in one or a few genomes.

We then analyzed the SDs that were only lost in Noctuoidea and Bombycoidea (Group B, Fig. 3b). There were 46 cases found in this group. As part of our comparative analyses, we also found some regions where duplication patterns were inconsistent with the generally accepted phylogeny (Groups C, D, and E; Fig. 3b). Such a scenario could arise as a result of a de novo development of SDs or as a result of deleted events, which might play a role in lineage-specific evolution. Groups C and D (Fig. 3b) were more common than the other groups due to their closer relationship with species based on the evolution history (Fig. 3b). The previous study of Marques-Bonet et al. [6] reports that humans share a greater number of SDs with chimpanzees than macaque or orangutan. Only 49 common SDs were lost in D. plexippus and H. melpomene (Group E). Since the time of Lepidoptera speciation is relatively long, we cannot test the complete phylogeny of SDs, and a greater number of sequenced Lepidoptera genomes would be necessary to elucidate this aspect.

We speculated that the “shared” SDs between species might represent the “ancestral sequences” as they remained conserved in the genomes during the evolution of Lepidoptera. To test this speculation, we analyzed the alignment identity of “shared SDs” and “unique SDs” and found that “shared SDs” had significantly higher identity comparing with the “unique SDs” (P < 0.01, T-test), except for silkworm (Fig. 3c). The results indicated that “shared SDs” might be more conserved than the “unique SDs”. Silkworm has diverged from the other species due to its domestication and inbreeding history leading to extremely low level of heterozygosity [36]. We compared the SDs in silkworm with the artificial selected regions that were identified in Xia et al. [36] and found that eight SD regions overlapped with the artificial selected regions, suggesting that these SDs may be related with the silkworm domestication. However, none of these eight regions were “shared SDs”, which also indicated that these unique SDs may be involved in the lineage-specific domestication. We also tested the difference of variance between “shared” and “unique” SDs (Fig. 3c) and showed that in P. xylostella, D. plexippus and H. melpomene, the differences were not significant (p = 0.05755; p = 0.5304 and p = 0.6278, respectively). Only B. mori and M. sexta showed significant differences (p = 1.218e-05 and p = 0.03909).

Sequence properties of the SDs in the five studied species

The analysis and comparison of the composition of genes in the SDs among the five Lepidoptera species showed that 2235, 1036, 1453, 1564 and 332 putative genes could be identified in P. xylostella, M. sexta, H. melpomene, D. plexippus and B. mori respectively (Table 1, Additional file 2: Table S2). Most of the segmental duplication intervals identified contained gene duplicates, ranging from 58% in silkworm to 94% in H. melpomene (Additional file 3: Table S3). We further characterized the genes as “shared” or “unique” based on whether they were located in the “shared SDs” or “unique SDs”. The results showed that most of the genes belonged to the “unique” genes, with only 31, 26, 13, 6, and 3 genes belonging to “shared” genes in P. xylostella, M. sexta, D. plexippus, H. melpomene, and B. mori, respectively. These results suggested that most of the genes in SDs could play different roles in different species. We hypothesized that these genes might be involved in lineage-specific evolution and particular gene classes might be overrepresented in the SDs.

To test the hypothesis, we used Gene Ontology (GO) to annotate all the genes and showed that each species had different GO enrichments and gene families (Table 2; Additional file 4: Table S4). In P. xylostella, 25 proteins were identified such as serine-type endopeptidase activity (GO: 0004252), structural constitute of cuticle (GO: 0042302), and nucleic acid binding (GO: 0003676) (Table 2). Based on previous study of differential expression in response to host-plant on Swedish comma, Polygonia c-album, these genes may be related to host-feeding [37, 38]. Thus, we suggested that the genes in SDs of diamondback moth might be related with its host-feeding behavior.

Table 2 GO enrichment for some proteins within the SDs regions among the five Lepidoptera species

In M. sexta, we identified a GO enrichment of prothoracicotrophic hormone activity (GO: 0018445). The prothoracicotropic hormone (PTTH) is well studied in tobacco hornworm (Rountree and Bollenbacher 1986) and in M. sexta, it is related to molting and metamorphosis [39, 40]. In D. plexippus, we identified the GO enrichment of glucuronosyltransferase activity (GO: 0015020). In silkworm, UDP-glucuronosyltransferase (UGT) plays a role in detoxification processes, such as minimizing the harmful effects of ingested plant allelochemicals [41]. Also, we identified the enrichment of Rho guanyl-nucleotide exchange factor activity (GO: 0005089), which is a modulator in the signaling pathway of Ras/MAPK and Wnt. Previous studies have shown that this activity is associated with neuronal growth cone and planar cell polarity formation [42, 43]. In B. mori, consistent with [30], we identified the enrichment of monooxygenase activity (GO: 0004497), which might be associated with detoxification.

To further clarify the functions of SDs in each Lepidoptera species, we annotated the gene functions in the SDs regions using Pfam and although the GO enrichments differed among species, some of the gene families embedded in the SDs were the same for the five species (Additional file 5: Table S5). For example, genes in SDs can be classified into three categories: (1) detoxification, (2) immunity, and (3) environmental signal recognition, which are similar to other mammals and insects [30, 44]. These genes are very important in drug detoxification, defense, and receptor and signal reorganization. The cytochrome P450s (P450s), for example, are important proteins for insect growth and development and have been found to play various functions such as biosynthesis of hormones, and inactivation and metabolism of xenobiotic compounds such as pesticides [45,46,47]. In this study, P450s were identified in all five species (8, 13, 15, 12, 10 SDs regions in P. xylostella, B. mori, M. sexta, D. plexippus and H. melpomene, respectively). In P. xylostella, Yu et al. [48] report strong expression of 84 functional cytochrome P450 genes, many of them, especially CYP367s, contributing to detoxification or metabolic processing of environmental chemicals.

We also identified the trypsin in the five species (55, 7, 24, 19, 2 SDs regions in P. xylostella, B. mori, M. sexta, D. plexippus and H. melpomene, respectively), which may be involved in immunity [49]. The glucose-methanol-choline (GMC) oxidoreductases, shown to be involved in developmental and physiological processes, and immunity [50], were also identified in four of the five species (1, 4, 5, 4 SDs regions in B. mori, M. sexta, D. plexippus and H. melpomene, respectively).

Some species-specific genes in SDs regions were also identified including 13 Lepidopteran-specific Lipoprotein_11 in silkworm. Zhang et al. [51] have shown that this family is involved in various physiological processes such as energy storage, embryonic development and immunity. These SDs might have played a role in the silkworm-specific evolution. Some lineage-specific expansion genes were also embedded in the SDs regions. For example, we identified 167 zinc-finger proteins in the SD regions of P. xylostella, which was much more than in any other species (20, 73, 91 and 8 in B. mori, M. sexta, D. plexippus and H. melpomene). A recent study (data unpubl.) of transcription factors in diamondback moth indicates that zinc-finger proteins may be expanded, also suggesting their potential important functions in the DBM. The zinc-finger has been shown to function in a variety of biological processes, such as DNA-binding, RNA-binding, protein-protein interactions, developmental processes and differentiation [52]. Further studies on expression patterns showed that the expression of some zinc-fingers were significantly different between susceptible and resistant strains (data unpublished). However, more researches are needed to illustrate the functions of these zinc-fingers.

Zhao et al. [30] report in silkworm that SDs are characterized by enrichment of DNA transposons and LTR retrotransposons. These observed enrichments in the flanking regions of SDs in silkworm suggest a potential implication in the formation of repeats in SDs. In this study, the TEs composition was analyzed by comparing the sequences near the SDs regions and found that DNA transposons were enriched in SDs regions as well as flanking regions of most species except H. melpomene (Table 3). Like in silkworm, DNA transposons and LTR (long terminal repeat) retrotransposons were enriched in the region of SDs and flanking regions in P. xylostella (Table 3), suggesting similar potential roles in SD formation. In M. sexta and D. plexippus, only DNA transposons were found to be enriched (Table 3). In H. melpomene, all analyzed TEs, except DNA transposons, were enriched in the SDs and flanking regions (Table 3) with LINEs (long interspersed nuclear elements) being the most abundant. These results suggest that short interspersed nuclear elements (SINEs), LTR and LINEs may also be involved in the formation of SDs in the genome of H. melpomene.

Table 3 TEs properties of the Lepidoptera genomes, duplications and 2.5 Kb flanking regions

Effects of SDs on gene expression

An initial study of lymphoblastoid cell lines in human has shown that CNVs have some effects on gene expression [53]. For example, changes in the number of copies can explain almost 20% of the variation in gene expression [53]. This effect can be the results of gene dosage within SDs or SDs on neighboring genes [53,54,55,56]. To assess the effect of SDs on the transcriptomes, we explored the genome-wide expression of three of the Lepidoptera species, P. xylostella, B. mori, and M. sexta, at different developmental stages, different tissues and different strains using RNA-seq data (NCBI website). We found that the gene expression levels embedded within our SDs regions were significantly lower than that of other genes located elsewhere in the genome. This was true for all analyzed available developmental stages or tissues (T-test, p < 2.20E-16) (Fig. 4). For example, in P. xylostella, we analyzed the expression pattern of genes within and outside the SD regions in different developmental stages. The results showed that the expression values of genes within SDs were significantly lower than the genes outside the SDs regions (T-test, p < 0.01, Fig. 4a). We redid the same analysis on the silk gland from different strains of B. mori and different tissues of M. sexta and found similar expression patterns: genes located in SDs had lower expression values than the genes outside SDs (Fig. 4b and c).

Fig. 4
figure4

Expression levels are associated with segmental duplications in the 5 species. a Expression level analysis in P. xylostella. Different developmental stages (from egg to adult) are listed. The expression level for the genes located in SDs is marked as red while the expression level for the genes outside SDs is marked as black. b Expression level analysis in M. sexta. Different tissues and developmental stages used in the analysis are listed. The expression level for the genes located in SDs is marked as green while the expression level for the genes outside SDs is marked as black. c Expression level analysis in silk gland of B. mori. Different strains were analyzed including domesticated strain Chunhua (D_CH), domesticated strain Chunyu (D_CY), wild silkworm Ankang from Baihe county of Shanxi Province (W_AKBH) and wild silkworm Ankang from Shiquan county of Shanxi Province (W_AKSQ). d Methylation level comparison between genes within and without SDs regions based on silkworm data

A possible reason for this may be that some regulation mechanisms control the gene expression within SDs. Based on our analysis above, we found that some TEs were enriched in the SDs as well as the SDs’ flanking regions (Table 3). In silkworm, methylation levels in TEs regions are extremely low compared to the rest of the genome [57]. Epigenetic regulation in insects can have various effects on biological processes. In silkworm, CG methylation is enriched in gene bodies and is positively correlated with gene expression level, indicating its positive roles in gene transcription [57]. We therefore analyzed the CG methylation level of the genes embedded in the SD regions as well as the genes outside the SDs regions for the five species and did not find any CG methylation in gene bodies of SDs (Fig. 4c). This may explain the low gene expression levels in SDs regions. However, more CG methylation information from other Lepidoptera species may be needed to further validate this conclusion.

Conclusion

Structural variation between genomes is important in phenotype differentiation and genome evolution. Here, we performed a comparative analysis of segmental duplications (SDs) among five lepidopteran reference genomes (P. xylostella, D. plexippus, B. mori, M. sexta and H. melpomene). We found that the SDs contents greatly varied among the five species. Comparative analyses of SDs showed that most of them arose after the divergence of each lineage. The most closely related species based on the phylogenetic tree also shared more common SDs. Conserved ancestral SDs and species specific SD events were assessed, revealing multiple examples of gain, loss or maintenance of SDs over time. The results indicated that SDs might have undergone loss or gain during the evolution of the genome. We further analyzed the genes embedded in SDs regions and the result showed that most of the genes were located in the species-specific SDs (“Unique” SDs). Functional analysis of these genes suggested their potential roles in the lineage-specific evolution. Comparison of gene expression between SDs and non-SDs showed that the expression levels of genes embedded in SDs were significantly lower, suggesting that structural changes in the genomes were involved in gene expression differences within each species. Our results suggested that SDs might have been involved in the species-specific evolution. They thus provide a valuable resource beyond the genetic mutation to explore the genome structure for future Lepidoptera research.

Abbreviations

CNV:

Copy number variations;

GO:

Gene Ontology

SD:

Segmental duplications

SSR:

Simple sequence repeats

TE:

Transposable elements

WGAC:

Whole-Genome Assembly Comparison

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Acknowledgements

We would like to extend our gratitude to Simon Wade Baxter, for his advice and useful suggestions on this study.

Funding

This work is supported by National Natural Science Foundation of China (No. 31320103922 and No. 31230061), Natural Science Foundation of Fujian Province (No. 2017 J0102). LV is supported by the “111” Program of SAFEA and the Minjiang Scholarships.

Availability of data and materials

All data generated during this study are included in this articles and its supplementary information.

Author information

QZ carried out data processing, analysis and wrote the manuscript; MY conceived and designed the study; DM helped to collect the data used in this study and LV helped draft the manuscript. All authors read and approved the final manuscript.

Correspondence to Minsheng You.

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Additional files

Additional file 1: Table S1.

SDs in the five studied genomes. (XLSX 1727 kb)

Additional file 2: Table S2.

Genes embedded in the SDs regions. (XLSX 81 kb)

Additional file 3: Table S3.

Gene duplicates located in the SD intervals. (XLSX 68 kb)

Additional file 4: Table S4.

GO enrichment for proteins within the SDs regions among the five studied Lepidoptera species. Only p < 0.01 were shown. (DOCX 20 kb)

Additional file 5: Table S5.

Gene families that were found to be different among the SDs regions from the five studied genomes. (XLSX 10 kb)

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Zhao, Q., Ma, D., Vasseur, L. et al. Segmental duplications: evolution and impact among the current Lepidoptera genomes. BMC Evol Biol 17, 161 (2017) doi:10.1186/s12862-017-1007-y

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Keywords

  • Segmental duplications
  • Lepidoptera
  • Evolution