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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: Minimal tool set for a prokaryotic circadian clock

Fig. 6

Activity of diverged KaiC homologs. a Conservation of important motifs in cyanobacterial KaiC1, KaiC2 and KaiC3 homologs based on WebLogo analyses. Motifs in KaiC1 homologs are displayed for proteins from organisms, which encode a KaiA protein or lack KaiA, respectively. Numbers indicate the residues in KaiC-7942. Properties of the residues are displayed as follows: polar (green), neutral (purple), basic (blue), acidic (red), and hydrophobic (black). b, c Phosphate uptake analyses of selected KaiC1 (b) and KaiC3 (c) homologs at 30 °C in dependence of KaiA. KaiC proteins were incubated with or without KaiA-7942 in the presence of γ-P32-ATP. After 0, 0.75, 1.5, 3, and 22 hours samples were separated via SDS-PAGE, stained with Coomassie (CBB), and subjected to autoradiography (ARG). The asterix indicates a contaminating protein. d Kinase activity of archaeal KaiC3 homologs at 75 °C. KaiC3 proteins from Thermococcus litoralis and Pyrococcus horikoshii were incubated with γ-P32-ATP and autophosphorylation was analyzed after 0, 5, 10, and 15 minutes. Shown are the Coomassie stained proteins (CBB) and autoradiography (ARG)

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