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Table 2 Characteristics of the nSSRs markers used in the Iberian populations of Brachypodium distachyon

From: Environmental isolation explains Iberian genetic diversity in the highly homozygous model grass Brachypodium distachyon

Locus

Repeat motif

Allele size range (bp)

Na

pnull

AR

Ho

Hs

HT

Gst

FIS

FIS §

ALB006

(GT)15

360–374

16

0.00399

3.318

0.000

0.051

0.614

0.917

1.000a

1.000

ALB022

(CT)11

354–358

14

0.00464

2.909

0.000

0.000

0.520

1.000

1.000

1.000

ALB040

(CTT)8

176–182

15

0.00416

1.975

0.024

0.031

0.169

0.819

0.221a

0.871

ALB050

(GT)15

217–231

19

0.00311

4.838

0.000

0.072

0.794

0.910

1.000a

1.000

ALB086

(AAG)7

190–198

14

0.00434

2.909

0.000

0.000

0.520

1.000

1.000

1.000

ALB087

(AGC)7

192–202

14

0.00355

2.909

0.000

0.000

0.520

1.000

1.000

1.000

ALB139

(AGA)7

308–310

14

0.00495

1.999

0.000

0.000

0.459

1.000

1.000

1.000

ALB165

(ATA)12

173–201

18

0.00369

4.745

0.000

0.099

0.776

0.873

1.000a

1.000

ALB181

(AC)9

234–238

14

0.00437

1.983

0.000

0.000

0.337

1.000

1.000

1.000

ALB311

(GA)6

244–250

14

0.00152

2.649

0.000

0.000

0.357

1.000

1.000

1.000

Overall

-

-

15

0.00383

3.023

0.002

0.025

0.507

0.952

0.922

0.987

  1. Na: total number of alleles; pnull: average frequency of null alleles across populations; AR: average allelic richness; Hs: expected within population Nei’s heterozygosity; Ho: observed within population Nei’s heterozygosity; HT: expected Nei’s heterozygosity within the total population; Gst: the Nei’s measure of genetic differentiation; FIS: inbreeding coefficient estimated in FSTAT (a indicates values deviating from HWE); FIS §: inbreeding coefficient estimated using the Bayesian procedure implemented in INEst)