Fig. 4

Phylogenies analysed for episodic selection. Each orthologous cluster was analysed by the Branch-site REL method. The shade of each colour on branches indicates strength of selection (red shows ω >13, blue ω ≤1 and grey ω = 1). The size of each colour represents the percentage of sites in the corresponding class found by Branch-site REL. Branches have been classified as undergoing episodic diversifying selection by the p-value corrected for multiple testing using the Holm-Bonferroni method at p < 0.05. a. clade 1; b. pviv/pcynmsp7B and 7E; c. pviv/pcynmsp7C and pinumsp7B; d. pviv/pcynmsp7F and pinumsp7C; e. pviv/pcynmsp7G, pkno/pcoamsp7C and pinumsp7D; f. pviv/pcynmsp7H/7I, pkno/pcoamsp7D and pinumsp7E; g. clade 2; h. pviv/pcynmsp7L and pinumsp7H and i. clade 4. At the bottom of each phylogeny there is a scale representation of msp7s. The blue boxes represent the encoded N-terminal region, the light brown ones symbolise the central region and the purple boxes the MSP_7C domain. Numbers within boxes represent the number of codons under positive selection inferred by MEME, SLAC, FEL, REL and FUBAR methods using the Datamonkey web server