Fine-scale assessment of genetic diversity of trembling aspen in northwestern North America

Table 3 Results of bottleneck analyses performed with the software Bottleneck version 1.2.02 (Cornuet and Luikart, [52]) and the program MPval [51] to calculate the M-ratio and M-critical with θ = 10 for each of the 27 populations

Population	He	P-TPM	P-SMM	P-IAM	M-Ratio^a	Mc^b
Pass	0,607	0,393	0.372	0.436	0,641	0,624
Morin Lake	0,663	0,374	0.058	0.339	0,615	0,696
Biggar	0,658	0,014	0.002	0.176	0,687	0,694
Peter Pond	0,636	0,366	0.613	0.297	0,665	0,647
Calling Lake	0,638	0,058	0.014	0.170	0,684	0,723
Red Earth	0,667	0,063	0.002	0.378	0,698	0,73
High Level	0,632	0,596	0.386	0.304	0,583	0,634
Ministik	0,617	0,563	0.443	0.272	0,642	0,671
Alders Flat	0,63	0,183	0.066	0.308	0,646	0,682
Hinton	0,659	0,175	0.060	0.182	0,748	0,71
Dunvegan	0,618	0,013	0.002	0.380	0,754	0,722
Dawson Creek	0,611	0,008	0.008	0.018	0,637	0,597
Fort Nelson	0,613	0,386	0.376	0.573	0,687	0,701
Liard Spring	0,662	0,373	0.002	0.629	0,705	0,714
Simpson Lake	0,658	0,057	0.012	0.383	0,6	0,647
Whitehorse	0,613	0,055	0.058	0.608	0,614	0,664
Taylor Hwy	0,655	0,057	0.013	0.398	0,704	0,676
Tok	0,644	0,014	0.002	0.355	0,653	0,723
Delta	0,657	0,063	0.051	0.397	0,716	0,655
Glennallen	0,658	0,056	0.000	0.376	0,723	0,73
Chena Park	0,682	0,631	0.395	0.595	0,656	0,676
Steese Hwy	0,656	0,059	0.015	0.372	0,791	0,767
Fairbanks	0,67	0,167	0.058	0.611	0,663	0,624
Richardson	0,633	0,017	0.002	0.065	0,682	0,634
Palmer	0,668	0,06	0.014	0.365	0,674	0,722
Kenai	0,674	0,368	0.173	0.382	0,699	0,688
Coldfoot	0,659	0,002	0.000	0.375	0,727	0,734

Values in bold show a significant bottleneck detected
For the heterozygosity excess test, we tested a stepwise mutation model, an infinite alleles model and a two-phase mutation model with 12 % multistep mutations and a variance = 0.36. M-ratio average was calculated across loci. Mc is the critical M-value calculated through the M-crit program developed by Garza and Williamson [51]. M-ratio test is significant if M-Ratio < Mc
^aM-Ratio = number of alleles/range in allele size and range (size of largest allele - size of smallest allele + 1)
^bMc is defined such that only 5 % of the simulation values fall below this threshold

ISSN: 2730-7182