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Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: Genotype diversity in the honey bee parasite Nosema ceranae: multi-strain isolates, cryptic sex or both?

Fig. 2

Partial comparison of direct sequencing results for X639 marker and cloned fragments. The chromatograms given above correspond to genotypes #1, #3 and #7, in the segment marked in the sequences of haplotypes (middle). Note that there are two double peaks (R) in Gt11 which are not ambiguous in Gt1 and Gt3 (G or A, but not both). Clones obtained from Gt11 had G or A in such points (below). Genotypes 1, 2, 3, and 4 were the only ones with no ambiguous points (see Fig. 1). Among the 38 clones analyzed, 15 haplotypes were obtained, including those identical to genotypes 1, 2, and 3, as well as several recombinant haplotypes and even two (#1 and #15) that were not expected from the sequence of genotype 11 and must represent minority sequences existing in the original isolate (see text). The nucleotides shadowed in the haplotypes correspond to the positions 159, 186 and 477 of the alignment

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