Fig. 6

Indirect immunofluorescence microscopy of daughter colony formation in Eudorina. Differential interference contrast (DIC) images (top row), fluorescence images (middle row) labeled with anti-SAS-6 (green) and anti-histone (magenta) antibodies, and merged images of DIC and fluorescence images (bottom row) of the same embryos are shown lengthwise. Scale bars: 5 μm. Positions of basal bodies labeled with anti-SAS-6 antibody (arrowheads) and chloroplasts (c) are shown. Note that basal bodies of daughter protoplasts are positioned in the center of concave surface of plakea before inversion (a–d). Basal bodies are moved from interior or concave surface of embryo to the outer surface of the spheroidal daughter colony by inversion (d–f). a Early 8-celled embryo. b Late 8-celled embryo. c Early 16-celled embryo. d Early inversion stage of 32-celled embryo. Note that the periphery of the plakea commences bending toward outside and enlarging the anterior opening of the plakea. e Inverting 32-celled embryo. f 32-celled embryo after inversion