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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: On the role of the proventricle region in reproduction and regeneration in Typosyllis antoni (Annelida: Syllidae)

Fig. 6

Attached (a-d) and detached (e-f) stolons of Typosyllis antoni. Anti-α-tubulin (glow mode, a-b) and anti-f-actin (depth coded, legend inserted in c, c-f) staining, confocal maximum projections. Anterior is left, all images are ventral views. Arrows in b indicate the border between stock and stolon, asterisks designate eyes, red dotted lines in d indicate edges of stock (anterior) and attached stolon (posterior) while white dotted lines in e and f indicate the anterior margin of the stolon. a Overview and b detailed image of marked area with reduced stack number. Near the detachment site and between the eyes, a dorsal orientated loop coming from the ventral nerve cord appeared, representing the stolon brain (sbr). The ventral nerve cords (vn; arrowheads) of stock and stolon were still connected. c Overview and d detailed image of marked area. The musculature is only weakly developed at the transition between the remaining body and the stolon. At the anterior margin of the stolon, the transverse musculature (tm) appears contracted. e Male stolon and f stolon of unknown sex. In front of a level between the parapodia of the stolon head, no musculature is visible. At this level the transverse musculature formed a sphincter muscle (ssm). Abbreviations: ga, gamete; sat, stolon antenna; sbr, stolon brain; ssm, stolon sphincter muscle; tm, transverse musculature; vlm, ventral longitudinal musculature; vn, ventral nerve cord. Scale bars = 100 μm (a, c, e), 50 μm (b, d, f)

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