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Table 2 Full model summaries for P2 and sperm features

From: Meiotic drive changes sperm precedence patterns in house mice: potential for male alternative mating tactics?

Model

Response variable

Random effects

Fixed effects

Mean (SD)

Fixed effect centred/

standardised?

Estimate

[approx. 95 % c.i.]

z value/

F value

p

GLMM

P2:

Male ID

Intercept (competition +/+ vs +/+)

  

−1.47 [−2.39, -0.54]

−3.21

0.001

 

Paternity share 2nd male

 

Male genotype combination (+/t vs +/t)

–

n/n

4.30 [2.55, 6.04]

4.99

< 0.001

   

Ejaculation number 1st male – 2nd male

0.3 (0.5)

n/n

−1.39 [−2.75, −0.04]

−2.08

0.038

   

Ejaculation interval 1st to 2nd male [h]

2.0 (0.9)

y/y

−1.39 [−2.12, −0.67]

−3.88

< 0.001

   

Weight difference 1st male – 2nd male

−1.3 (2.2)

y/y

0.22 [−0.42, 0.86]

0.70

0.484

   

Female genotype (28 +/+; 12 +/t)

–

y/n

1.33 [−0.03, 2.69]

1.96

0.048

LMM

PC1:

Brother pair/Male ID

Intercept (centred for genotype)

  

0.32 [0.15, 0.48]

–

–

 

Progressive sperm speed

Incubation time*Male ID

t haplotype

–

y/n

−0.35 [−0.66, −0.04]

4.44

0.048

   

Incubation time [h]

2.5 (2.0)

n/n

−0.05 [−0.07, −0.02]

9.80

0.005

   

Sperm count

71.3 (36.7)

y/y

−0.28 [−0.38, −0.17]

23.60

< 0.001

   

t haplotype*Time

–

–

0.01 [−0.05, 0.06]

0.04

0.850

   

t haplotype*Sperm count

–

–

0.05 [−0.08, 0.18]

0.43

0.517

   

Sperm count*Time

–

–

0.01 [−0.02, 0.03]

0.40

0.528

LMM

PC2:

Brother pair/Male ID

Intercept (centred for genotype)

  

0.27 [0.08, 0.47]

–

–

 

Sperm head speed

Incubation time*Male ID

t haplotype

–

y/n

0.12 [−0.18, 0.41]

0.53

0.476

   

Incubation time [h]

2.5 (2.0)

n/n

−0.10 [−0.13, −0.08]

48.10

< 0.001

   

Sperm count

71.3 (36.7)

y/y

−0.09 [−0.22, 0.03]

1.75

0.190

   

t haplotype*Time

–

–

−0.06 [−0.11, 0.003]

3.07

0.096

   

t haplotype*Sperm count

–

–

0.10 [−0.06, 0.27]

1.17

0.285

   

Sperm count*Time

–

–

0.005 [−0.03, 0.04]

0.06

0.809

  1. LMM linear mixed model, GLMM generalised linear mixed model. Intercepts correspond to estimates for wild type (+/+) males (paternity share) or were centered for male genotype (sperm features) by assigning values of -0.5 and +0.5 to +/+ and +/t males, respectively. Thus, intercepts in the sperm models correspond to an average between +/+ and +/t males for an incubation time of 0 h, and t haplotype shows the change in sperm features for +/t relative to +/+ males. Approximate 95 % confidence intervals were obtained by multiplying Student’s t-values for our sample sizes by standard errors of the predicted values [60]. 95 % confidence intervals not overlapping zero and p values < 0.05 are highlighted in bold. Degrees of freedom for F values were based on the Kenward-Roger approximation [58]