Fine-scale genetic structure among greater sage-grouse leks in central Nevada
© The Author(s). 2016
Received: 8 February 2016
Accepted: 7 June 2016
Published: 14 June 2016
Mating systems that reduce dispersal and lead to non-random mating might increase the potential for genetic structure to arise at fine geographic scales. Greater sage-grouse (Centrocercus urophasianus) have a lek-based mating system and exhibit high site fidelity and skewed mating ratios. We quantified population structure by analyzing variation at 27,866 single-nucleotide polymorphisms in 140 males from ten leks (within five lek complexes) occurring in a small geographic region in central Nevada.
Lek complexes, and to a lesser extent individual leks, formed statistically identifiable clusters in ordination analyses, providing evidence for fine-scale geographic genetic differentiation. Lek geography predicted genetic differentiation even at a small geographic scale, which could be sharpened by strong site fidelity. Relatedness was also higher among individuals within lek complexes (and leks), suggesting that reproductive skew, where few males participate in most of the successful matings, could also potentially contribute to genetic differentiation. Models incorporating a habitat resistance surface as a proxy for potentially reduced movement due to landscape features indicated that both geographic distance and habitat suitability (i.e. preferred habitat) predicted genetic structure, with no significant effect of man-made barriers to movement (i.e. power lines and roads). Finally, we illustrate how data sets containing fewer loci (<4000) had less statistical precision and failed to detect the full degree of genetic structure.
Our results suggest that habitat features and lek site geography of sage-grouse shape fine scale genetic structure, and highlight how larger data sets can have increased precision and accuracy for quantifying ecologically relevant genetic structure over small geographic scales.
Population genetic studies are essential for understanding how behavioral, ecological, and evolutionary processes shape the geographic distribution of genetic variation. Recent DNA sequencing advances now allow variation to be readily assayed at thousands of loci for large numbers of individuals and populations in non-model organisms [1, 2], potentially illuminating the relationships among ecological, genetic, and geographic variation at previously unattainable scales [3–6]. These approaches have value for uncovering previously unrecognized patterns of genetic structure, generating more precise estimates of demographic and evolutionary parameters, and providing increased opportunity for understanding adaptation [7–10]. Such approaches also have special promise for analyses of genetic variation related to contemporary ecological processes and population demography in species of ecological or conservation significance [7, 11, 12].
Variation in behavioral and life history traits may have important consequences for the geographic structuring of genetic variation across populations (e.g. [13–16]). For example, a number of avian species have lek-based mating systems in which a small number of males contribute disproportionately to reproduction, dispersal is restricted by strong site fidelity, and leks (i.e. mating arenas) remain at the same geographic locations for long periods of time [17–21]. Strong site fidelity could reduce gene flow among leks and give rise to spatial genetic structure even in the absence of geographic barriers. Because a subset of males typically contribute disproportionately to reproduction on leks, reproductive skew (i.e. where few males participate in most of the successful matings) can also lower effective population size (N e) and increase genetic drift [17, 22, 23]. These factors, in addition to the potential for kin selection in lek-based mating systems [24, 25], could enhance the effects of drift in structured populations, give rise to fine-scale spatial genetic structure, and increase extinction probability of isolated populations [17, 26–29].
Greater sage-grouse (Centrocercus urophasianus; hereafter sage-grouse) are an iconic western North American bird species with a lek-based mating system . Male sage-grouse display for females at leks residing in open patches of sagebrush (Artemesia spp.), where a subset of dominant males can account for a large proportion of the successful matings (; but see ). Degradation of sagebrush ecosystems across North America has substantially reduced the geographic range and abundance of sage-grouse over the last century. Population declines ranged from 45 to 90 % across the distribution and local extirpations have occurred in many regions [33, 34]. Habitat degradation and population declines [35, 36] have raised concerns about population persistence (but see  for an alternative viewpoint), though sage-grouse were recently found unwarranted for listing under the U.S. Endangered Species Act . Low movement rates among leks due to high site fidelity (e.g. ), in addition to reproductive skew, could interact with habitat variation to limit gene flow, enhance genetic drift, and shape fine-scale genetic structure in sage-grouse populations, as has been found in several lek-breeding species (e.g. [17, 20, 40]).
Previous studies utilizing small data sets of traditional molecular markers (e.g. mtDNA; microsatellites) have reported variable patterns of genetic structure across sage-grouse populations, leaving uncertainty about the scale of geographic genetic structure and obscuring the expected link between demographic and population genetic parameters across the range . The detection of genetic differentiation across broad geographic scales resulted in the recognition of three distinct populations: Gunnison sage-grouse (C. minimus), greater sage-grouse, and the Bi-state population of greater sage-grouse (spanning the California and Nevada border) [42–44]. However, most studies at finer geographic scales have not identified genetic structure among geographically proximate leks [45, 46], leaving uncertainty about the potential for site fidelity and reproductive skew to influence genetic structure [45, 47, 48]. In contrast, a recent study of geographically proximate leks in northwestern Colorado documented among-lek differentiation and evidence for isolation by distance . Although these studies suggest movements among leks may limit differentiation in some parts of the range, higher resolution data sets should improve our understanding of the geographic scale of genetic structure and the factors associated with it.
Sage-grouse sample collection
During the spring breeding season, we sampled ten leks separated by an average distance of 35.7 km in Eureka County, Nevada (Fig. 1; Additional file 1: Table S1) with known histories of individual movements and sufficiently large sample sizes . Although 4–5 additional leks occur within this immediate area, the sampled leks have been the subject of past behavioral and ecological studies, remained constantly active from 2003 to 2012, and were evenly distributed across the region . A priori, we grouped leks into five lek complexes based on geographic proximity (<15 km), patterns of male movements documented across multiple years of monitoring, and potential habitat barriers . Observed movements among leks were highly uncommon (only 1.5 % of 355 males moved between leks), but typically occurred within the same lek complex . Two state highways, unpaved secondary roads, and power transmission lines are present in the study area, but sage-grouse regularly crossed these infrastructures (based on 10 years of radio-telemetry data; DG, EJB, and JSS, unpublished data). There are no major geographic barriers to dispersal in the study area, but it is possible that individuals are less likely to move through low-quality habitat. Sage-grouse here make regular seasonal movements from spring breeding leks to higher elevation summer/fall habitat (>2100 m): individuals from the Pine Valley, Kobeh Valley, and Pony Express lek complexes utilize the Roberts Creek Range, while individuals from the Cortez and North Cortez lek complexes typically occupy the Cortez Range [50, 51]. Although birds from different leks and lek complexes share summer habitat and can have large yearly and summer movement distances (Additional file 1: Figure S1), nearly all birds return to their specific lekking grounds each spring . Thus, the sage-grouse in our study area exhibit strong lek-site fidelity.
We captured 140 male sage-grouse on these leks during the spring over a 5 year period (March–May, 2007–2012). We only included males in our study because we were unable to collect a sufficient number of female samples. Approximately 1.5 ml of blood was drawn from the basilic vein of each captured bird. Protocols for the capture and handling of sage-grouse were approved by the University of Nevada, Reno Institutional Animal Care and Use Committee (Protocol Numbers A02/03-22, A05/06-22, A07/08-22, A09/10-22).
Genotyping-by-sequencing library preparation
DNA was extracted from blood using Qiagen DNeasy Blood and Tissue kits (Qiagen Inc., Valencia, CA) and quantified using a Nanodrop spectrophotometer (Thermo Scientific Inc., Waltham, MA). Reduced-representation libraries were constructed using a GBS approach [52, 53]. Genomic DNA was first cut with two restriction enzymes, EcoRI and MseI, and a unique DNA barcode was ligated to digested fragments from each individual. Each EcoRI adaptor consisted of bases matching the endonuclease cut site, a unique eight to ten base pair DNA barcode sequence, and an Illumina adaptor; the MseI adaptors consisted of the endonuclease cut site and the opposite Illumina adaptor. Uniquely barcoded ligation products from all individuals were pooled and PCR-amplified using standard Illumina primers. Libraries were size-selected for a region between 350 and 450 bases using a BluePippin quantitative electrophoresis unit (Sage Science, Beverly, MA). We generated single end, 100 bp reads on one lane of an Illumina HiSeq 2500 platform at the University of Texas Genomic Sequencing and Analysis Facility (Austin, TX).
Assembly and variant calling
Illumina reads were first filtered to remove potential contaminant DNA (PhiX, E. coli) and low quality or aberrant reads. We used a Perl script to recognize barcodes assigned to each individual bird, to correct single-base errors in barcode sequences, and to remove sequences containing portions of the Illumina adaptors. After removing barcodes and cut site bases, retained sequences were 87, 88, or 89 bp in length. A reference of GBS sequenced regions was created with de novo assembly of a random subset of 25 million reads using the SeqMan ngen software (DNASTAR, Inc.). This used a minimum match percentage of 95, a gap penalty of 30, and only retained contigs containing a minimum of 10 reads (additional assembly parameters are available from the authors by request). We generated the reference by removing contigs that were over-assembled or that were shorter than 84 or longer than 90 bases. Reads from each bird were subsequently aligned to the reference using the aln and samse algorithms in bwa (Burrows-Wheeler Aligner; ).
The number of reads representing alternative nucleotide states (i.e. SNPs) at each variant site was quantified using samtools and bcftools . We only called SNPs when 90 % of the individuals had at least one read at the locus. Genotype likelihoods were calculated with bcftools, stored in Variant Call Format, and converted to a composite genotype likelihood format for downstream analysis. We excluded variable sites with more than one alternate allele and loci with minor allele frequencies less than 5 %. For contigs containing more than one SNP, we randomly selected a single SNP to increase the independence of loci used in subsequent analyses. Assembly related files and genotype matrices are available at Dryad (doi:10.5061/dryad.652r5), and additional parameter settings for these analyses are available from the authors by request.
We used a hierarchical Bayesian model that incorporates uncertainty in sequencing coverage across loci and individuals to estimate allele frequencies and genotype probabilities simultaneously for all individuals based on estimated genotype likelihoods . This model treats allele frequencies as priors, and coincidentally estimates allele frequencies and genotype probabilities while incorporating uncertainty arising from variation in sequence coverage. Thus, the estimates incorporate information on the probability of sampling each of the two alleles at a locus from the population, as well as coverage. We obtained posterior estimates of genotype probabilities by running 10,000 MCMC steps after a 6000 step burn-in and thinning every other step. These genotype probabilities were then converted to composite genotype values, where an individual’s genotype ranged from 0 to 2 at a locus (values of 0 and 2 represent homozygotes for different alleles, while values of 1 represent heterozygotes).
The relationship between geography and genetic structure
To summarize genotypic variation across individuals and to explore the relationship between geography and genetic structure, we utilized three complementary ordination methods . For all three approaches, we implemented permutational multivariate analysis of variance (PERMANOVA; ) in the vegan package  in R  as a post hoc test of differentiation among leks and lek complexes based on Euclidean distances of the first two ordination axes.
We first conducted principal component analysis (PCA) on genotype probabilities using the prcomp function in R. To quantify the relationship between individual genotypic variation and geography, we conducted Procrustes analyses on principal components (PCs) [60, 61] using the vegan package in R. We rotated a genomic matrix of PC1 and PC2 onto a geographic matrix of latitudes and longitudes and used 999 permutations to test significance of Procrustes correlations. To test the hypothesis that sage-grouse within a lek complex are more genetically similar to one another than expected, we calculated the Euclidean genetic distance among all individuals using the first two PCs. For each subset, the observed genetic distance of individual pairs sharing the same lek complex was compared to a null distribution of genetic distances constructed from 1000 permutations of lek complex-sharing status, which was randomly assigned among pairs. The same analyses were conducted at the lek level.
We conducted Discriminant Analysis of PCs (DAPC)  using the adegenet package [63, 64] in R. Unlike PCA, DAPC maximizes the ratio between the variance explained between groups and the total variance explained  and is often used to group individuals into clusters. DAPC is useful for GBS datasets because the analysis effectively reduces the dimensionality of large datasets while delineating population genetic structure in a somewhat analogous manner to more computationally intensive Bayesian clustering methods (e.g. STRUCTURE; ) . To reduce over- or under-discrimination, we selected the number of retained PCs by predicting the maximum α-score with the optim.a.score function (20 replicate α-scores were calculated), following . We first conducted DAPC without a priori group assignments by inferring the most likely number of genetic clusters (K) using the find.clusters function in the adegenet package. This function utilizes K-means clustering to calculate a Bayesian information criterion (BIC) value for each potential value of K (the most likely K has the lowest BIC value) and delineates individual group assignments for DAPC . Additionally, we conducted DAPC using a priori group assignments at both the lek (K = 10) and lek complex (K = 5) scales, which were based on patterns of male movement across multiple years of monitoring .
We additionally used redundancy analysis (RDA)  to assess the relationship between geography and genetic structure. RDA is a constrained ordination analogous to multivariate regression in which a predictor dataset (in this case, lek geography) is used to maximize the variance partitioned in a response dataset (sage-grouse genotype probabilities) . RDA has been previously employed to compare the relative strengths of geography and other potential barriers to gene flow (e.g. climate, habitat) as predictors of population genetic structure (e.g. [67, 68]). We performed RDA with latitude and longitude as predictors of our genotype probability matrix using the vegan package in R. The correlation between lek latitude and longitude was−0.396 (R), which is smaller than the maximum correlation of RDA predictor variables (|R| = 0.7) suggested by .
Genetic structure and habitat resistance
To further summarize genetic differentiation among leks, we calculated pairwise F ST  based on allele frequencies within leks across all loci, and assessed significance using a permutation approach. As an additional metric of differentiation, we calculated Nei’s genetic distance (D; ) and used these values to generate neighbor-joining trees with the ape package  in R.
To assess if environmental features contribute to observed differentiation among leks, we used a landscape genetic approach that compared genetic distance (D) with the summed environmental resistance (a proxy for potentially reduced movement due to unsuitable habitat or barriers) between each pair of leks using a least-cost paths framework . We derived a habitat resistance surface by taking the inverse of female sage-grouse nest site habitat selection model developed for this system  (see Fig. 1b). Additionally, we developed resistance surfaces including anthropogenic features (e.g. highways and transmission lines) as barriers at different thresholds of movement permeability. Movement barrier resistance surfaces were derived from a 100 m buffer around all roads and power lines within the study area. As outlined by , each pixel within 100 m of a barrier was assigned either the maximum environmental resistance value from the habitat model (permeable barriers) or assigned a resistance value four orders of magnitude greater than the maximum resistance value (low-permeable barriers). For each resistance surface, we used the landscape genetics toolbox  in ArcMap 10.0 (Environmental Systems Research Institute, Redlands, CA) to produce a pairwise matrix of least-cost distance paths among leks. We assessed the association between genetic distance and least-cost path for each resistance surface with Mantel tests calculated in the ecodist package  in R.
The effect of genomic sampling on genetic structure estimates
To determine the number of SNPs required to capture geographic genetic structure and to examine the robustness of our results to the number of loci included in our analyses, we performed power analyses for PCA and DAPC. We constructed ten randomly sampled datasets of 22 different sizes, ranging from 50 to 27,000 SNPs (220 datasets in total). For each dataset, we conducted PCA and Procrustes analysis as described above. Additionally, we employed DAPC on each subset to determine how data set size influenced the proportions of individuals correctly assigned to lek and lek complex.
We also evaluated the possible influence of loci with exceptionally high F ST (i.e., “outlier” loci) on genetic structure parameter estimates by generating genotype sets with highly differentiated loci removed. We generated locus-specific pairwise F ST estimates  for all 45 pairwise combinations of leks and for all ten pairwise combinations of lek complexes. We removed loci with F ST estimates above the 98th and 97th quantiles of the genome-wide F ST distribution for any pairwise analysis at the lek and lek complex levels, and conducted PCA and Procrustes analyses on each of the four trimmed data sets.
After quality screening, we retained 169,622,388 DNA sequences from 140 individuals. The initial de novo assembly placed 22,175,595 reads into a set of 218,483 high-coverage contigs; the consensus sequences of these contigs were then used as a reference for the genomic regions represented by our GBS data. The final assembly contained 14 × 107 reads (85 % of total reads) with an average of 838,715 reads assembled per individual. After identifying variant sites where >90 % of individuals had at least one read, we retained 27,866 SNPs with minor allele frequencies >5 % and with an average coverage of 5.7X per locus per individual (sd = 1.7). Analyses below are based on genotype probabilities estimated with the hierarchical Bayesian model of .
The relationship between geography and genetic structure
For the DAPC without a priori group assignments, two PCs were retained based on 20 replicate α-score tests (mean = 1.80; sd = 1.43). The K = 2 model fit the data best; BIC steadily increased from K = 2 to K = 40 (Additional file 1: Figure S4A). The first discriminant function (DF) clearly separated sage-grouse individuals into two clusters (Additional file 1: Figure S4B); one group mostly comprised individuals from the North Cortez and Cortez lek complexes, while the other group was composed of individuals from the Pine Valley, Pony Express, and Kobeh Valley lek complexes (Additional file 1: Figure S4C).
Genetic structure and habitat resistance
Summary results from Mantel models testing if genetic distance (D; ) among sage-grouse leks is predicted by geographic distance, habitat suitability, and potential barriers to movement (e.g. roads and power lines)
Distance + habitata
Distance + permeable barriersb
Distance + low-permeable barriersb
The effect of genomic sampling on genetic structure estimates
We also examined how the results displayed in Fig. 2 (PCA; Procrustes; latitude vs. PC1 regression) were affected by removing loci with exceptionally high F ST estimates. After removing loci with F ST estimates above the 97th and 98th quantile for all pairwise lek comparisons, subsets of 22,615 and 16,630 loci were retained, while subsets of 25,991 and 23,040 loci were retained after the lek complex comparisons. Results from PCA, Procrustes, and regression analyses based on the four “outlier” trimmed genotype subsets were indistinguishable from those for the full data set (Additional file 1: Figure S5).
Our results indicate that lek geography and habitat features influence sage-grouse population genetic variation at a fine geographic scale across the individual, lek, and lek complex levels. Consistent with isolation by lek distance, geography explained a large proportion of individual genetic variation in ordination space (Figs. 2 and 4), predetermined delineations of lek complexes clustered together (Figs. 3 and 5), and lek-level estimates of genetic distance were strongly related to geographic distance (Table 1; Fig. 6). Although statistically significant, levels of genetic differentiation among neighboring leks were low, as indicated by the low percentage of variation explained by the first two axes of PCA, DAPC, and RDA and by the small estimates of genetic differentiation among leks (Fig. 5). Such low levels of differentiation are not surprising and are consistent with past studies based on smaller datasets not detecting genetic differentiation across geographically proximate leks (e.g. [43, 45, 47, 77]).
Sage-grouse from the different lek complexes formed distinct and statistically identifiable clusters in ordination space. The most pronounced genetic structure divided northern lek complexes (Cortez and North Cortez) from more southern complexes (Kobey Valley, Pony Express, and Pine Valley) (Figs. 2, 3 and 4, Additional file 1: Figure S4). The DAPC analysis without a priori information inferred K = 2 as the best model and assigned individuals almost entirely to these two groups (Additional file 1: Figure S4). This pattern is consistent with sage-grouse from the Cortez and North Cortez complexes predominantly sharing summer brood rearing habitat in the Cortez Range, while individuals from other complexes typically cohabitate in the Roberts Creek Range [50, 51]. Nonetheless, individuals are highly faithful to their specific leks during subsequent spring breeding seasons . Additionally these results are in accord with other studies reporting that the pattern and strength of genetic structure can depend on which stage in the breeding cycle samples are collected (in this case, mating vs. brood rearing) (e.g. ). Within these groups, a priori designated lek complexes formed largely non-overlapping clusters in ordination analyses (Figs. 2, 3 and 4), with ~80 % of individuals assigned to the correct lek complex in DAPC (Fig. 3). Differentiation among neighboring leks was much less pronounced; only ~49 % of individuals were assigned to the correct lek in DAPC, and individuals from neighboring leks overlapped substantially in ordination space. Even with limited gene flow among neighboring leks, our results illustrate a hierarchical pattern of fine-scale spatial genetic structure consistent with isolation by effective lek and lek complex distance (Fig. 6).
The strong site fidelity previously documented in this system  could reduce gene flow among leks at some geographic scales. Capture-mark-recapture data from these leks indicate that inter-lek movements by males are very rare (~1.5 %; ) even though individuals have overlapping summer distributions [50, 51] and can move large distances across the landscape within a year (Additional file 1: Figure S1). By using geographic distances among leks in our population-level genetic analyses we more directly accounted for the effect of lek location on genetic structure. Nearly all of the movements we did observe were among neighboring leks, which could contribute to limited differentiation within complexes (e.g. ). Our landscape genetic analyses based on habitat resistance surfaces indicate that habitat suitability also influences genetic distance among leks and could reinforce the effect of site fidelity on hierarchical genetic structure at the lek and lek complex levels (Table 1). Similar to , we did not find evidence of an influence of anthropogenic barriers on genetic distance among leks, even though certain structures within this system (i.e. transmission lines) were associated with reduced population growth . These results contrast with another study that documented significant impacts of anthropogenic barriers on population genetic structure in sage-grouse from the state of Washington .
Reproductive skew caused by a subset of males contributing disproportionately to reproduction  could also potentially influence population differentiation by reducing effective population sizes and reducing the impact of migration on genetic differentiation . Empirical evidence consistent with reproductive skew and/or kin selection has been found for other bird species with lek-based or cooperative mating systems (e.g. [16, 20, 40, 80–82]); however, the strength of kin selection can be context-dependent . Previous analyses of sage-grouse have not found evidence for increased relatedness within leks (e.g. ). Nevertheless, sage-grouse that shared lek complexes (and leks) in our study were more genetically similar than would be expected by chance (Additional file 1: Figure S2), a pattern one would predict if reproductive skew were influencing geographic genetic variation. Although it is difficult to directly account for the effects of reproductive skew and kin selection on levels of population genetic structure, our results suggest that the lek-breeding system could potentially influence the hierarchical pattern of genetic differentiation we observe. Indeed, social and reproductive behaviors are commonly associated with genetic structure in vertebrates and insects [15, 84–86]. As habitat fragmentation and reproductive skew could enhance differentiation and lead to loss of variation, such mating systems could pose special challenges for protecting genetic diversity in declining species.
Our analyses provide a finer view of geographic genetic structure than most previous studies of sage-grouse (reviewed by ), although some studies of lek-mating birds have detected a subtle influence of lek geography (e.g. [17, 18]). In most previous studies, genetic differentiation among sage-grouse populations has been documented when leks have been isolated by large expanses of low-quality habitat (e.g. [44, 47, 77, 88]). Studies at smaller geographic scales have typically recovered less fine-scale structure, but have found evidence for isolation by distance (e.g. ) and, in one case, genetic differentiation among groups of leks found in neighboring management zones . It is possible that the strength of genetic structure in our study was elevated as a result of either excluding females (e.g. if females exhibit lower lek fidelity than males; ) or including leks that occupy a part of the range where site fidelity, relatedness, and genetic differentiation interact at different levels. However, our ability to detect such fine-scale genetic structure was likely influenced by the much larger number of loci employed in our study (but see ).
Not surprisingly, we found that the level of genetic structure detected and the precision of population genetic parameter estimates initially increased as the number of loci analyzed rose (Fig. 7). Larger numbers of loci (>4000) more successfully captured the relationship between genetic variation and geography (Fig. 3) and increased the fraction of individuals assigned to the correct lek and lek complex in discriminant analyses (Fig. 3). However, parameter estimates and their precision were consistent in randomly built data sets with >4000 loci (Fig. 7). In addition, parameter estimates in our analyses of the full data set of SNPs were indistinguishable from those for data sets with high F ST outlier loci removed. This indicates that the overall patterns in our results were not strongly influenced by loci with exceptional patterns of differentiation or residing in particular genomic locations. Our results are consistent with many recent studies that illustrate how the information content of population genetic analyses can depend on the extent of genomic sampling (e.g. [89–91]). For instance, a recent study of Soay sheep found that the precision and accuracy of heritability estimates grew with increasing marker numbers (reaching an asymptote at ~18,000 SNPs), and parameter estimates including this many markers were comparable to results calculated using pedigrees .
Along with other recent studies detecting previously unrecognized structure, our results demonstrate the promise of population genomic data sets for quantifying fine-scale, ecologically relevant population genetic variation [90, 93]. The increase in resolution such approaches could bring is highly relevant to the field of conservation biology  because the discovery of cryptic genetic structure in species of conservation concern has the potential to inform management strategies (e.g. [90, 95]). Indeed, the fine-scale geographic genetic structure of numerous species may be shaped in part by behavioral processes, including habitat selection (e.g. ), site fidelity (e.g. ), and reproductive skew (e.g. ). Future investigations utilizing genome-level data sets and covering wider ecological and geographic contexts should help to tease apart the influence of habitat features, behavioral ecology, and gene flow in shaping fine-scale genetic variation in natural populations.
BIC, bayesian information criterion; DAPC, discriminant analysis of principal components; DF, discriminant function; GBS, genotyping-by-sequencing; PC, principal component; PCA, principal component analysis; PERMANOVA, permutational multivariate analysis of variance; RDA, redundancy analysis; SNP, single nucleotide polymorphism
We thank Matthew Cronin, Matt Forister, David McDonald, Chris Nice, Vladimir Pravosudov, the University of Nevada, Reno EECB peer review group, and one anonymous reviewer for providing comments and discussion that improved the manuscript. We also thank numerous field technicians and volunteers for assistance with sage-grouse capture and monitoring.
This research was funded by: University of Nevada Agricultural Experiment Station; Nevada Department of Wildlife; U.S. Bureau of Land Management; National Fish and Wildlife Foundation; NV Energy Corp; Transformative Research Grant from the Graduate Program in Ecology, Evolution, and Conservation Biology at the University of Nevada, Reno. JPJ was supported by National Science Foundation DEB-1145609. CLW was supported by National Science Foundation Graduate Research Fellowship Program DGE-1447692.
Availability of data and materials
A directory containing the Illumina library prep protocol, compressed alignment.bam files for each individual, and a matrix of genotype probabilities used for downstream analyses are deposited at Dryad (doi:10.5061/dryad.652r5).
All authors designed the study. DG and EJB collected sage-grouse material from the field. JPJ, DG, CLW, and TLP performed the molecular work, including DNA extractions and library construction for genotyping-by-sequencing. JPJ, DG, and TLP analyzed the data and wrote the initial draft, but all authors edited and revised the final version of the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Ethics approval and consent to participate
Protocols for the capture and handling of sage-grouse were approved by the University of Nevada, Reno Institutional Animal Care and Use Committee (Protocol Numbers A02/03-22, A05/06-22, A07/08-22, A09/10-22).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Hohenlohe PA, Day MD, Amish SJ, Miller MR, Kamps-Hughes N, Boyer MC, et al. Genomic patterns of introgression in rainbow and westslope cutthroat trout illuminated by overlapping paired-end rad sequencing. Mol Ecol. 2013;22:3002–13.View ArticlePubMedPubMed CentralGoogle Scholar
- Narum SR, Buerkle CA, Davey JW, Miller MR, Hohenlohe PA. Genotyping-by-sequencing in ecological and conservation genomics. Mol Ecol. 2013;22:2841–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Deagle BE, Jones FC, Chan YF, Absher DM, Kingsley DM, Reimchen TE. Population genomics of parallel phenotypic evolution in stickleback across stream-lake ecological transitions. Proc R Soc B. 2012;279:1277–86.View ArticlePubMedGoogle Scholar
- Leslie S, Winney B, Hellenthal G, Davison D, Boumertit A, Day T, et al. The fine-scale genetic structure of the British population. Nature. 2015;519:309–14.View ArticlePubMedPubMed CentralGoogle Scholar
- Novembre J, Johnson T, Bryc K, Kutalik Z, Boyko AR, Auton A, et al. Genes mirror geography within Europe. Nature. 2008;456:98–101.View ArticlePubMedPubMed CentralGoogle Scholar
- Ralph P, Coop G. The geography of recent genetic ancestry across Europe. PLoS Biol. 2013;11:e1001555.View ArticlePubMedPubMed CentralGoogle Scholar
- Allendorf FW, Hohenlohe PA, Luikart G. Genomics and the future of conservation genetics. Nat Rev Genet. 2010;11:697–709.View ArticlePubMedGoogle Scholar
- Berg JJ, Coop G. A population genetic signal of polygenic adaptation. PLoS Genet. 2014;10:e1004412.View ArticlePubMedPubMed CentralGoogle Scholar
- Gompert Z, Comeault AA, Farkas TE, Feder JL, Parchman TL, Buerkle CA, et al. Experimental evidence for ecological selection on genome variation in the wild. Ecol Lett. 2014;17:369–79.View ArticlePubMedGoogle Scholar
- Soria-Carrasco V, Gompert Z, Comeault AA, Farkas TE, Parchman TL, Johnston JS, et al. Stick insect genomes reveal natural selection’s role in parallel speciation. Science. 2014;344:738–42.View ArticlePubMedGoogle Scholar
- Avise JC. Perspective: conservation genetics enters the genomics era. Conserv Genet. 2010;11:665–9.View ArticleGoogle Scholar
- Funk WC, McKay JK, Hohenlohe PA, Allendorf FW. Harnessing genomics for delineating conservation units. Trends Ecol Evol. 2012;27:489–96.View ArticlePubMedPubMed CentralGoogle Scholar
- Coltman DW, Pilkington JG, Pemberton JM. Fine-scale genetic structure in a free-living ungulate population. Mol Ecol. 2003;12:733–42.View ArticlePubMedGoogle Scholar
- Jonker RM, Kraus RHS, Zhang Q, van Hooft P, Larsson K, van der Jeugd HP, et al. Genetic consequences of breaking migratory traditions in barnacle geese Branta leucopsis. Mol Ecol. 2013;22:5835–47.View ArticlePubMedGoogle Scholar
- Lecomte N, Gauthier G, Giroux JF, Milot E, Bernatchez L. Tug of war between continental gene flow and rearing site philopatry in a migratory bird: the sex-biased dispersal paradigm reconsidered. Mol Ecol. 2009;18:593–602.View ArticlePubMedGoogle Scholar
- van Dijk RE, Covas R, Doutrelant C, Spottiswoode CN, Hatchwell BJ. Fine-scale genetic structure reflects sex-specific dispersal strategies in a population of sociable weavers (Philetairus socius). Mol Ecol. 2015;24:4296–311.View ArticlePubMedGoogle Scholar
- Bouzat JL, Johnson K. Genetic structure among closely spaced leks in a peripheral population of lesser prairie-chickens. Mol Ecol. 2004;13:499–505.View ArticlePubMedGoogle Scholar
- Francisco MR, Gibbs HL, Galetti M, Lunardi VO, Galetti PM. Genetic structure in a tropical lek-breeding bird, the blue manakin (Chiroxiphia caudata) in the Brazilian Atlantic Forest. Mol Ecol. 2007;16:4908–18.View ArticlePubMedGoogle Scholar
- Höglund J, Alatalo RV. Leks. Princeton: Princeton University Press; 1995.View ArticleGoogle Scholar
- Höglund J, Alatalo RV, Lundberg A, Rintamäki PT, Lindell J. Microsatellite markers reveal the potential for kin selection on black grouse leks. Proc R Soc B. 1999;266:813–6.View ArticleGoogle Scholar
- Wiley RH. Territoriality and non-random mating in sage grouse, Centrocercus urophasianus. Anim Behav Monogr. 1973;6:85–99.View ArticleGoogle Scholar
- Mills LS, Allendorf FW. The one-migrant-per-generation rule in conservation and management. Conserv Biol. 1996;10:1509–18.View ArticleGoogle Scholar
- Stiver JR, Apa AD, Remington TE, Gibson RM. Polygyny and female breeding failure reduce effective population size in the lekking Gunnison sage-grouse. Biol Conserv. 2008;141:472–81.View ArticleGoogle Scholar
- Höglund J. Lek-kin in birds – provoking theory and surprising new results. Ann Zool Fennici. 2003;40:249–53.Google Scholar
- Kokko H, Lindstrom J. Kin selection and the evolution of leks: whose success do young males maximize? Proc R Soc B. 1996;263:919–23.View ArticleGoogle Scholar
- Johnson JA, Bellinger MR, Toepfer JE, Dunn P. Temporal changes in allele frequencies and low effective population size in greater prairie-chickens. Mol Ecol. 2004;13:2617–30.View ArticlePubMedGoogle Scholar
- McDonald DB. Microsatellite DNA, evidence for gene flow in neotropical lek-mating long-tailed manakins. Condor. 2003;105:580–6.View ArticleGoogle Scholar
- Segelbacher G, Höglund J, Storch I. From connectivity to isolation: genetic consequences of population fragmentation in capercaillie across Europe. Mol Ecol. 2003;12:1773–80.View ArticlePubMedGoogle Scholar
- Westemeier RL, Brawn JD, Simpson SA, Esker TL, Jansen RW, Walk JW, et al. Tracking the long-term decline and recovery of an isolated population. Science. 1998;282:1695–8.View ArticlePubMedGoogle Scholar
- Connelly JW, Hagen CA, Schroeder MA. Characteristics and dynamics of greater sage-grouse populations. Stud Avian Biol. 2011;38:53–68.Google Scholar
- Wiley RH. Lekking in birds and mammals: behavioral and evolutionary issues. Adv Study Behav. 1991;20:201–91.View ArticleGoogle Scholar
- Bird KL, Aldridge CL, Carpenter JE, Paszkowski CA, Boyce MS, Coltman DW. The secret sex lives of sage-grouse: multiple paternity and intraspecific nest parasitism revealed through genetic analysis. Behav Ecol. 2013;24:29–38.View ArticleGoogle Scholar
- Connelly JW, Braun CE. Long-term changes in sage grouse Centrocercus urophasianus populations in western North America. Wildl Biol. 1997;3:229-34.Google Scholar
- Schroeder MA, Aldridge CL, Apa AD, Bohne JR, Braun CE, Bunnell SD, et al. Distribution of sage-grouse in North America. Condor. 2004;106:363–76.View ArticleGoogle Scholar
- Aldridge CL, Nielsen SE, Beyer HL, Boyce MS, Connelly JW, Knick ST, et al. Range-wide patterns of greater sage-grouse persistence. Divers Distrib. 2008;14:983–94.View ArticleGoogle Scholar
- Garton EO, Connelly JW, Horne JS, Hagen C, Moser A, Schroeder MA. Greater sage-grouse population dynamics and probability of persistence. Stud Avian Biol. 2011;38:293–382.Google Scholar
- Cronin MA. The greater sage-grouse story: do we have it right? Rangelands. 2015;37:200–4.View ArticleGoogle Scholar
- U.S. Fish and Wildlife Service. Endangered and threatened wildlife and plants; 12-month finding on a petition to list greater sage-grouse (Centrocercus urophasianus) as an endangered or threatened species. Fed Reg. 2015;80:59858–942.Google Scholar
- Gibson D, Blomberg EJ, Atamian MT, Sedinger JS. Lek fidelity and movement among leks by male greater sage-grouse centrocercus urophasianus: a capture-mark-recapture approach. Ibis. 2014;156:729–40.View ArticleGoogle Scholar
- Petrie M, Krupa A, Burke T. Peacocks lek with relatives even in the absence of social and environmental cues. Nature. 1999;401:155–7.View ArticleGoogle Scholar
- Zink RM. Comparison of patterns of genetic variation and demographic history in the greater sage-grouse (Centrocercus urophasianus): relevance for conservation. Open Ornithol J. 2014;7:19–29.View ArticleGoogle Scholar
- Oyler-McCance SJ, Kahn NW, Burnham KP, Braun CE, Quinn TW. A population genetic comparison of large- and small-bodied sage grouse in Colorado using microsatellite and mitochondrial DNA markers. Mol Ecol. 1999;8:1457–65.View ArticlePubMedGoogle Scholar
- Oyler-McCance SJ, Taylor SE, Quinn TW. A multilocus population genetic survey of greater sage-grouse across their range. Mol Ecol. 2005;14:1293–310.View ArticlePubMedGoogle Scholar
- Oyler-McCance SJ, Casazza ML, Fike JA, Coates PS. Hierarchical spatial genetic structure in a distinct population segment of greater sage-grouse. Conserv Genet. 2014;15:1299–311.View ArticleGoogle Scholar
- Bush KL, Aldridge CL, Carpenter JE, Paszkowski CA, Boyce MS, Coltman DW. Birds of a feather do not always lek together: genetic diversity and kinship structure of greater sage-grouse (Centrocercus urophasianus) in Alberta. Auk. 2010;127:343–53.View ArticleGoogle Scholar
- Davis DM, Reese KP, Gardner SC, Bird KL. Genetic structure of greater sage-grouse (centrocercus urophasianus) in a declining, peripheral population. Condor. 2015;117:530–44.View ArticleGoogle Scholar
- Bush KL, Dyte CK, Moynahan BJ, Aldridge CL, Sauls HS, Battazzo AM, et al. Population structure and genetic diversity of greater sage-grouse (Centrocercus urophasianus) in fragmented landscapes at the northern edge of their range. Conserv Genet. 2011;12:527–42.View ArticleGoogle Scholar
- Gibson RM, Pires D, Delaney KS, Wayne RK. Microsatellite DNA analysis shows that greater sage-grouse leks are not kin groups. Mol Ecol. 2005;14:4453–9.View ArticlePubMedGoogle Scholar
- Thompson TR. Dispersal ecology of greater sage-grouse in Northwestern Colorado: evidence from demographic and genetic methods. PhD dissertation. Moscow: University of Idaho; 2012.Google Scholar
- Atamian MT, Sedinger JS, Heaton JS, Blomberg EJ. Landscape-level assessment of brood rearing habitat for greater sage-grouse in Nevada. J Wildl Manag. 2010;74:1533–43.View ArticleGoogle Scholar
- Blomberg EJ, Gibson D, Sedinger JS, Casazza ML, Coates PS. Intraseasonal variation in survival and probable causes of mortality in greater sage-grouse Centrocercus urophasianus. Wildl Biol. 2013;19:347–57.View ArticleGoogle Scholar
- Gompert Z, Lucas LK, Nice CC, Fordyce JA, Forister ML, Buerkle CA. Genomic regions with a history of divergent selection affect fitness of hybrids between two butterfly species. Evolution. 2012;66:2167–81.View ArticlePubMedGoogle Scholar
- Parchman TL, Gompert Z, Mudge J, Schilkey F, Benkman CW, Buerkle CA. Genomewide association genetics of an adaptive trait in lodgepole pine. Mol Ecol. 2012;21:2991–3005.View ArticlePubMedGoogle Scholar
- Li H, Durbin R. Fast and accurate short read alignment with burrows–wheeler transform. Bioinformatics. 2009;25:1754–60.View ArticlePubMedPubMed CentralGoogle Scholar
- Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, et al. The sequence alignment/map format and SAMtools. Bioinformatics. 2009;25:2078–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Jombart T, Pontier D, Dufour AB. Genetic markers in the playground of multivariate analysis. Heredity. 2009;102:330–41.View ArticlePubMedGoogle Scholar
- Anderson MJ. A new method for non-parametric multivariate analysis of variance. Austral Ecol. 2001;26:32–46.Google Scholar
- Oksanen J, Blanchet FG, Kindt R, Legendre P, Minchin PR, O’Hara RB, et al. vegan: community ecology package. R package version 2.0-9; 2015.Google Scholar
- R Core Team. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL http://www.r-project.org/; 2015.
- Wang C, Szpiech ZA, Degnan JH, Jakobsson M, Pemberton TJ, Hardy JA, et al. Comparing spatial maps of human population-genetic variation using procrustes analysis. Stat Appl Genet Molec Biol. 2010;9:13.Google Scholar
- Wang C, Zöllner S, Rosenberg NA. A quantitative comparison of the similarity between genes and geography in worldwide human populations. PLoS Genet. 2012;8:e1002886.View ArticlePubMedPubMed CentralGoogle Scholar
- Jombart T, Devillard S, Balloux F. Discriminant analysis of principal components: a new method for the analysis of genetically structured populations. BMC Genet. 2010;11:94.View ArticlePubMedPubMed CentralGoogle Scholar
- Jombart T. adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics. 2008;24:1403–5.View ArticlePubMedGoogle Scholar
- Jombart T, Ahmed I. adegenet 1.3.1: new tools for the analysis of genome-wide SNP data. Bioinformatics. 2011;27:3070–1.View ArticlePubMedPubMed CentralGoogle Scholar
- Pritchard JK, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data. Genetics. 2000;155:945–59.PubMedPubMed CentralGoogle Scholar
- van den Wollenberg AL. Redundancy analysis an alternative for canonical correlation analysis. Psychometrika. 1977;42:207–19.View ArticleGoogle Scholar
- Hecht BC, Matala AP, Hess JE, Narum SR. Environmental adaptation in Chinook salmon (Oncorhynchus tshawytscha) throughout their North American range. Mol Ecol. 2015;24:5573–95.View ArticlePubMedGoogle Scholar
- Szulkin M, Gagnaire PA, Bierne N, Charmantier A. Population genomic footprints of fine-scale differentiation between habitats in Mediterranean blue tits. Mol Ecol. 2016;25:542–58.View ArticlePubMedGoogle Scholar
- Hudson RR, Slatkin M, Maddison WP. Estimation of levels of gene flow from DNA sequence data. Genetics. 1992;132:583–9.PubMedPubMed CentralGoogle Scholar
- Nei M. Genetic distance between populations. Am Nat. 1972;106:283–92.View ArticleGoogle Scholar
- Paradis E, Claude J, Strimmer K. APE: analyses of phylogenetics and evolution in R language. Bioinformatics. 2004;20:289–90.View ArticlePubMedGoogle Scholar
- Adriaensen F, Chardon JP, De Blust G, Swinnen E, Villalba S, Gulinck H, et al. The application of ‘least-cost’ modelling as a functional landscape model. Landscape Urban Plan. 2003;64:233–47.View ArticleGoogle Scholar
- Gibson D. The role of environmental stochasticity on population demography of greater sage-grouse in central Nevada, U.S.A. PhD dissertation. Reno: University of Nevada; 2015.Google Scholar
- Shirk AJ, Schroeder MA, Robb LA, Cushman SA. Empirical validation of landscape resistance models: insights from the greater sage-grouse (centrocercus urophasianus). Landscape Ecol. 2015;30:1837–50.View ArticleGoogle Scholar
- Etherington TR. Python based GIS tools for landscape genetics: visualising genetic relatedness and measuring landscape connectivity. Methods Ecol Evol. 2011;2:52–5.View ArticleGoogle Scholar
- Goslee SC, Urban DL. The ecodist package for dissimilarity-based analysis of ecological data. J Stat Softw. 2007;22:1–19.View ArticleGoogle Scholar
- Oyler-McCance SJ, Cornman RS, Jones KL, Fike JA. Genomic single-nucleotide polymorphisms confirm that Gunnison and greater sage-grouse are genetically well differentiated and that the Bi-state population is distinct. Condor. 2015;117:217–27.View ArticleGoogle Scholar
- Row JR, Oyler-McCance SJ, Fike JA, O’Donnell MS, Doherty KE, Aldridge CL, et al. Landscape characteristics influencing the genetic structure of greater sage-grouse within the stronghold of their range: a holistic modeling approach. Ecol Evol. 2015;5:1955–69.View ArticlePubMedPubMed CentralGoogle Scholar
- Schroeder MA, Robb LA. Fidelity of greater sage-grouse Centrocercus urophasianus to breeding areas in a fragmented landscape. Wildl Biol. 2003;9:291–9.Google Scholar
- Concannon MR, Stein AC, Uy JAC. Kin selection may contribute to lek evolution and trait introgression across an avian hybrid zone. Mol Ecol. 2012;21:1477–86.View ArticlePubMedGoogle Scholar
- Krakauer AH. Kin selection and cooperative courtship in wild turkeys. Nature. 2005;434:69–72.View ArticlePubMedGoogle Scholar
- Shorey L, Piertney S, Stone J, Höglund J. Fine-scale genetic structuring on Manacus manacus leks. Nature. 2000;408:352–3.View ArticlePubMedGoogle Scholar
- Lebigre C, Alatalo RV, Soulsbury CD, Höglund J, Siitari H. Limited indirect fitness benefits of male group membership in a lekking species. Mol Ecol. 2014;23:5356–65.View ArticlePubMedGoogle Scholar
- Archie EA, Maldonado JE, Hollister-Smith JA, Poole JH, Moss CJ, Fleischer RC, et al. Fine-scale population genetic structure in a fission-fusion society. Mol Ecol. 2008;17:2666–79.View ArticlePubMedGoogle Scholar
- Foitzik S, Rüger MH, Kureck IM, Metzler D. Macro- and microgeographic genetic structure in an ant species with alternative reproductive tactics in sexuals. J Evol Biol. 2011;24:2721–30.View ArticlePubMedGoogle Scholar
- Ross KG. Molecular ecology of social behaviour: analyses of breeding systems and genetic structure. Mol Ecol. 2001;10:265–84.View ArticlePubMedGoogle Scholar
- Oyler-McCance SJ, Quinn TW. Molecular insights into the biology of greater sage-grouse. Stud Avian Biol. 2011;38:85–94.Google Scholar
- Schulwitz S, Bedrosian B, Johnson JA. Low neutral genetic diversity in isolated greater sage-grouse (centrocercus urophasianus) populations in northwest Wyoming. Condor. 2014;116:560–73.View ArticleGoogle Scholar
- Benestan L, Gosselin T, Perrier C, Sainte-Marie B, Rochette R, Bernatchez L. RAD genotyping reveals fine-scale genetic structuring and provides powerful population assignment in a widely distributed marine species, the American lobster (Homarus americanus). Mol Ecol. 2015;24:3299–315.View ArticlePubMedGoogle Scholar
- Larson WA, Seeb LW, Everett MV, Waples RK, Templin WD, Seeb JE. Genotyping by sequencing resolves shallow population structure to inform conservation of Chinook salmon (Oncorhynchus tshawytscha). Evol Appl. 2014;7:355–69.View ArticlePubMedPubMed CentralGoogle Scholar
- Wagner CE, Keller I, Wittwer S, Selz OM, Mwaiko S, Greuter L, et al. Genome-wide RAD sequence data provide unprecedented resolution of species boundaries and relationships in the Lake Victoria cichlid adaptive radiation. Mol Ecol. 2013;22:787–98.View ArticlePubMedGoogle Scholar
- Bérénos C, Ellis PA, Pilkington JG, Pemberton JM. Estimating quantitative genetic parameters in wild populations: a comparison of pedigree and genomic approaches. Mol Ecol. 2014;23:3434–51.View ArticlePubMedPubMed CentralGoogle Scholar
- Corander J, Majander KK, Cheng L, Merilä J. High degree of cryptic population differentiation in the Baltic Sea herring Clupea harengus. Mol Ecol. 2013;22:2931–40.View ArticlePubMedGoogle Scholar
- McMahon BJ, Teeling EC, Höglund J. How and why should we implement genomics into conservation? Evol Appl. 2014;7:999–1007.View ArticlePubMedPubMed CentralGoogle Scholar
- McDonald DB, Parchman TL, Bower MR, Hubert WA, Rahel FJ. An introduced and a native vertebrate hybridize to form a genetic bridge to a second native species. Proc Natl Acad Sci U S A. 2008;105:10842–7.View ArticleGoogle Scholar