- Research article
- Open Access
Chromosomal phylogeny of Vampyressine bats (Chiroptera, Phyllostomidae) with description of two new sex chromosome systems
© The Author(s). 2016
Received: 29 October 2015
Accepted: 23 May 2016
Published: 4 June 2016
The subtribe Vampyressina (sensu Baker et al. 2003) encompasses approximately 43 species and seven genera and is a recent and diversified group of New World leaf-nosed bats specialized in fruit eating. The systematics of this group continues to be debated mainly because of the lack of congruence between topologies generated by molecular and morphological data. We analyzed seven species of all genera of vampyressine bats by multidirectional chromosome painting, using whole-chromosome-painting probes from Carollia brevicauda and Phyllostomus hastatus. Phylogenetic analyses were performed using shared discrete chromosomal segments as characters and the Phylogenetic Analysis Using Parsimony (PAUP) software package, using Desmodontinae as outgroup. We also used the Tree Analysis Using New Technology (TNT) software.
The result showed a well-supported phylogeny congruent with molecular topologies regarding the sister taxa relationship of Vampyressa and Mesophylla genera, as well as the close relationship between the genus Chiroderma and Vampyriscus.
Our results supported the hypothesis that all genera of this subtribe have compound sex chromosome systems that originated from an X-autosome translocation, an ancestral condition observed in the Stenodermatinae. Additional rearrangements occurred independently in the genus Vampyressa and Mesophylla yielding the X1X1X2X2/X1X2Y sex chromosome system. This work presents additional data supporting the hypothesis based on molecular studies regarding the polyphyly of the genus Vampyressa and its sister relationship to Mesophylla.
The subtribe Vampyressina (sensu Baker et al. 2003 ) corresponds to a diversified group of New World leaf-nosed bats specialized in fruit eating. This group encompasses 43 species of phyllostomid genera Platyrrhinus, Vampyrodes, Uroderma, Chiroderma, Vampyriscus, Vampyressa and Mesophylla [2–6]. Intergeneric relationships have been the focus of great debate, with disagreements mainly due to discrepant topologies generated by morphological and molecular data regarding the generic status of Vampyressa and Vampyriscus and their relationships with Chiroderma, Ectophylla and Mesophylla [1, 7–9].
Most studies based on morphological data support the sister taxa relationship between the genera Ectophylla and Mesophylla [7, 10, 11]. However, a study based on cranio-dental characters agrees with the molecular consensus about the close affinity of the genera Mesophylla and Vampyressa . On the other hand, a study of restriction site variation of mitochondrial genes ND3 and ND4 supports the morphological view . A reanalysis based on a direct survey on DNA sequences of these genes agrees with the molecular hypothesis .
Cytogenetic studies are agreed on the close relationship between Mesophylla and Vampyressa. All were based on gross chromosome morphology and the shared sex chromosome systems [14–17]. However, no cladistics analysis was made taking into account the different karyotypes of the species within this group.
Chromosome rearrangements are rare genomic changes (sensu ) and, because of their Mendelian inheritance, can be used for phylogenetic inferences. Phylogenetic studies based on chromosome data have contributed to the systematics of many groups of vertebrates, especially in Chiroptera [19–26].
In this paper, we use multidirectional chromosome painting with whole chromosome probes from two phyllostomid bats (Carollia brevicauda and Phyllostomus hastatus), as well as chromosome banding and Fluorescence In Situ Hybridization (FISH) with 18S rDNA and telomeric probes, to establish a genome-wide comparative chromosomal map for all genera of Vampyressine bats. Using chromosomal rearrangements as characters, we built a phylogeny that sheds some new light on the evolutionary relationships among these bats.
Species analyzed in this work and from Pieczarka et al. (2005, 2013) and Sotero-Caio et al. (2011)
Platyrrhinus incarum, PIN
Belém (1° 27’ 08” S; 48° 29’ 28” W)
Vampyrodes caraccioli, VCA
Belém (1° 27’ 08” S; 48° 29’ 28” W)
Chiroderma villosum, CVI
Juruti (2° 09’ 18” S; 56° 05’ 50” W) and Santarem (2° 26’ 57” S; 54° 41’ 59” W)
1 M, 1 F
Mesophylla macconnelli, MMA
Faro (1° 13’ 01” S; 57° 44’ 03” W) and Cotriguaçu (9° 52’ 10” S; 58° 33’ 18” W)
2 M, 1 F
Vampyressa thyone, VTH
Campos Novos (2° 22’ 12” N; 61° 26’ 08” W)
1 M, 1 F
Vampyriscus bidens, VBI
Belém (1° 27’ 08” S; 48° 29’ 28” W) and Faro (1° 13’ 01” S; 57° 44’ 03” W)
2 M, 1 F
Vampyriscus brocki, VBR
Lourenço (2° 19’ 49” N; 51° 37’ 08” W)
Uroderma magnirostrum, UMA
Pieczarka et al. (2013)
Uroderma bilobatum, UBI
Pieczarka et al. (2013)
Artibeus obscurus, AOB
Pieczarka et al. (2013)
Phyllostomus hastatus, PHA
Pieczarka et al. (2005)
Carollia brevicauda, CBR
Pieczarka et al. (2005)
Diphylla ecaudata, DEC
Sotero-Caio et al. (2011)
Diaemus youngi, DYO
Sotero-Caio et al. (2011)
Desmodus rotundus, DRO
Sotero-Caio et al. (2011)
Chromosomal preparation and chromosome banding
Metaphase spreads were obtained from bone marrow preparations after colchicine treatment, following  and , as well as from fibroblasts cultured according to . G-banding was performed using trypsin treatment  and subsequent incubation in saline solution (0.5 × SSC) at 60 °C, and staining with Wright’s solution . C-banding was carried out according to  and Ag-NOR staining followed .
Fluorescence in Situ Hybridization (FISH)
FISH using telomeric probes (All Human Telomere Probes, Oncor) and 18S rDNA probes from Prochilodus argenteus  labeled with biotin or digoxigenin by nick translation was performed. Whole-chromosome-specific painting probes from Phyllostomus hastatus (PHA) and Carollia brevicauda (CBR) were obtained from flow-sorted chromosomes , labeled by DOP-PCR (Degenerate Oligonucleotide-Primed-Polymerase Chain Reaction) amplification  and hybridized as previously described [25, 36]. After hybridization and washing, metaphases were stained with DAPI (4’,6-diamidino-2-phenylindole), and images captured using the Axiovision 3.0 software (Carl Zeiss) through an AxioCam MRm coupled to a Zeiss-Axiophot 2 microscope.
Data from literature
In previous publications [25, 37] the species Phyllostomus hastatus, Carollia brevicauda, Artibeus obscurus, Uroderma bilobatum and U. magnirostrum were analyzed using the same whole-chromosome probes as used here. We added those data to the present work, and included them in our phylogenetic analysis. Sotero-Caio et al.  analyzed Diphylla ecaudata, Diaemus youngi and Desmodus rotundus also using the same probes. These species (Desmondontinae) were used as outgroup on phylogenetic analysis because of their basal position in published molecular phylogenies and because this is a monophyletic subfamily with well supported molecular  and chromosomal  synapomorphies.
A data matrix was established where the chromosomal rearrangements are the characters under analysis, and discrete chromosomal syntenic blocks are the characters states that reflect the occurrence of rearrangements (Additional file 1: Table S1). The data matrix also included species previously analyzed [25, 37, 38], and it is available as a supplementary file. Maximum parsimony (MP) analysis was made using PAUP 4.0b10 . A heuristic search to find most parsimonious tree(s) was performed using Tree Bisection Reconnection (TBR) branch-swapping; the bootstrap posterior probability was obtained with one thousand replicates. The Bremer support or “decay index” [40, 41] was calculated to verify the inconsistency of the branches in the consensus tree using the software “Tree Analysis Using New Technology” (TNT) version 1.1 , freely distributed by the Willi Hennig Society.
Karyotype description and multidirectional chromosome painting in Chiroderma villosum (CVI)
Multidirectional chromosome painting using whole-chromosome probes from Carollia brevicauda (CBR) and Phyllostomus hastatus (PHA) revealed 25 and 23 shared conserved syntenic segments, respectively, with the number of syntenic blocks ranging from one to four on each chromosome of CVI (Fig. 1, CVI).
Karyotype description and multidirectional chromosome painting in Vampyriscus bidens (VBI)
All five specimens of V. bidens (Table 1) collected at different sites in the Brazilian Amazon have a 2n = 26 and FN = 48, with all chromosomes biarmed. The X is subtelocentric and the Y is acrocentric (Fig. 1, VBI). The CH is located at the centromeric region of chromosomes and in the proximal region of the Y chromosome (Additional file 2: Figure S1, VBI). Hybridization with telomeric probes showed signals at the tips of chromosomes and weaker signals at the centromeric regions of some chromosomes in association with the CH (Additional file 3: Figure S2, VBI). FISH using 18S rDNA probes, and silver staining, showed two NOR located on pairs 8 and 9 (Additional file 3: Figure S2, VBI).
Chromosome painting using CBR and PHA probes revealed 25 and 23 conserved segments on V. bidens, respectively. The number of syntenic blocks ranges from one to three in different VBI chromosomes (Fig. 1, VBI).
Karyotype description and multidirectional chromosome painting in Vampyriscus brocki (VBR)
Vampyriscus brocki collected in Amapá State has a 2n = 24 and a FN = 44 (Fig. 1, VBR). Its karyotype, as in the congeneric VBI, has only biarmed elements (meta, submeta and subtelocentric). The X is subtelocentric and the Y is acrocentric. The CH is located in the pericentromeric regions of chromosomes and the proximal part of Y (Additional file 2: Figure S1, VBR). FISH signals with telomeric probes were detected at the tips of chromosomes (Additional file 3: Figure S2, VBR). Signals from an 18S rDNA probe were detected at the distal portion of the short arm of pairs 5, 7, 8, and 9; in agreement with silver staining results (Additional file 3: Figure S2, VBR).
Chromosome painting using CBR and PHA probes detect 25 and 23 syntenic segments, respectively. We found a maximum of five segments per chromosome with CBR probes and four with PHA (Fig. 1, VBR).
Karyotype description and multidirectional chromosome painting in Vampyressa thyone (VTH)
Two specimens of V. thyone caught from Serra of Apeú in Roraima state were karyotyped and show a 2n = 24 complement in the female and 2n = 23 in the male, with FN = 20 (Fig. 1, VTH). The chromosome complement has only uniarmed pairs, and the sex chromosome system is X1X1X2X2/X1X2Y, so there is one chromosome less in the male karyotype. The CH is located in small bands in the pericentromeric regions of chromosomes, with one strong CH block in the interstitial part of ×2 (Additional file 2: Figure S1, VTH). FISH using the telomeric probe showed signals at the tips of chromosomes (Additional file 3: Figure S2, VTH); the 18S rDNA probe revealed two NORs on pairs 5 and 9 (Additional file 3: Figure S2, VTH), confirming previous results from silver nitrate (AgNOR) staining.
Chromosome painting revealed 28 and 29 shared segments with CBR and PHA probes, respectively. Results from each set of probes showed a maximum of four syntenic blocks per chromosome in VTH (Fig. 1, VTH).
Karyotype description and multidirectional chromosome painting in Mesophylla macconnelli (MMA)
We analyzed three specimens of M. macconnelli. The diploid number is 2n = 22 in female and 2n = 21 in males, with the FN = 18 (Fig. 1, MMA). The chromosomal complement has only uniarmed pairs. The sex chromosome system is X1X1X2X2/X1X2Y. The CH is present on small bands in the pericentromeric region of the autosomes and on the proximal part of the Y (Additional file 2: Figure S1, MMA). Probes revealed telomeric signals on the distal ends of all chromosomes (Additional file 3: Figure S2, MMA). Staining with silver nitrate (Ag-NOR) and FISH with 18S rDNA probe showed one signal in the distal portion of pair 2 (Additional file 3: Figure S2, MMA).
Chromosome painting on MMA metaphases revealed 28 syntenic segments using probes from CBR, and 30 syntenic segments using probes from PHA. We found a maximum of four segments per chromosome matching CBR probes, and five matching PHA probes (Fig. 1, MMA).
Karyotype description of Platyrrhinus incarum (PIN) and Vampyrodes caraccioli (VCA)
We analyzed single specimens of P. incarum and V. caraccioli. Both species have a 2n = 30, FN = 56 complement and a Neo-XY sex chromosome system (Additional file 4: Figure S3, PIN and VCA, respectively). The CH is located in the pericentromeric region of all chromosomes for PIN (Additional File 2: Figure S1, PIN) and in the centromeric regions of the chromosomal complement of VCA (Additional file 2: Figure S1, VCA); additional heterochromatic blocks are present at the distal ends of the short arms of chromosomes 5, 7 and 13 in both species. In PIN, FISH with telomeric probes produced signals at the tips of chromosomes and in the centromeric regions of pair 13 for both species. Additionally, minor signals in some pairs co-located with CH for PIN (Additional file 3: Figure S2, PIN). In VCA, telomeric probes detect signals at the tips of chromosomes and the centromeric regions of pairs 1, 5 and 13 (Additional file 3: Figure S2, VCA). Probe signals from 18S rDNA are located in pair 7, confirming the silver staining in PIN (Additional file 3: Figure S2, PIN), and in chromosome pairs 5 and 7 in VCA.
The banding patterns of these two species (PIN and VCA) are similar to those of Artibeus obscurus (Additional file 4: Figure S3 . The only differences found are a pericentric inversion on pair 5 in PIN, the multiple sex chromosome system in A. obscurus (XX/XY1Y2) and the Neo-XY in PIN and VCA. Because of the similar G-banded karyotypes in PIN, VCA, and AOB, we infer that the syntenic groups would be the same as in AOB.
Phylogenetic analyses using chromosome as characters
Genera Platyrrhinus and Vampyrodes
There are only two recognized species in Vampyrodes , whereas Platyrrhinus has at least 21 currently described species [5, 43, 44]. Both genera have the same 2n = 30 and FN = 56 karyotype, as well as the Neo-XY sex chromosome system (found in all species described thus far [14, 15, 45]). G-banding patterns have been published only for Platyrrhinus.
In our phylogeny, P. incarum and V. caracciolli share a polytomy along with the branch corresponding to the genera Vampyriscus and Chiroderma. This polytomy of P. incarum and V. caracciolli can be explained by their similarity in G-banding pattern and presence of only some autapomorphies. Since these species have no synapomorphies, their precise positions on the phylogeny cannot be determined, despite molecular studies suggest a close relationship among Vampyrodes, Platyrrhinus, Vampyriscus and Chiroderma . The chromosomal homeology among these species and Artibeus obscurus supports the hypothesis that the 2n = 30–31 karyotype is probably the ancestral condition for the Stenodermatinae . We found only two characteristics supporting the close relationship of Platyrrhinus and Vampyrodes, the shared NOR location observed on pair 7 and an interstitial telomeric site found on pair 13. These characteristics were not included in our phylogeny due to the variability of these marks in the Stenodermatinae. Because of the retention of several ancestral characters and the NOR position, we conclude that the genera Platyrrhinus and Vampyrodes probably are sister taxa, in accordance with most studies based on either the morphological or molecular data [7, 9, 43, 47].
Inter- and intraspecific variation in the genus Uroderma
Uroderma shows wide karyotypic variation when compared with other species in Stenodermatinae. Three chromosomal races have been described for U. bilobatum (Baker et al. 1972 , 1975 ). Specimens with 2n = 42 and FN = 50 occur in South America, east of the Andes (2n = 42 race). Individuals with 2n = 38 and FN = 44 are distributed on the Atlantic side of Guatemala, Honduras and Yucatan Peninsula of Mexico (Central America) and along the Pacific coast of Colombia and northern Ecuador (2n = 38 race). Specimens with 2n = 44 and FN = 48 occur in the Pacific coast of Central America in El Salvador, Guatemala, Honduras and México (2n = 44 race). A hybrid zone was found in Honduras, but introgression between the two races is low (Hoffmann et al. 2008 ). This hybrid zone has been extensively studied since the second half of the 20th century and there is major controversy regarding primary and secondary origins of the zone, the role of selective force in its maintenance, and the amount of gene flow across this zone (Baker et al. 1972 , Hoffmann et al. 2008 ).
Our data support the hypothesis that the 2n = 42 race is distributed throughout South and Central America. Geographic barriers such as the Andean mountains and probably the Gulf of Fonseca (which was under water throughout much of the Pleistocene) separated the race with 42 chromosomes, and gave rise to the 44 and 38 cytotypes (Hoffmann et al. 2008 ). There is temporal agreement between the geographic barrier and divergence of the three races that happened around 0.9 to 0.2 Mya (Hoffmann et al. 2008 ). Baker et al. (1982 ) have proposed the rearrangements that took place to form the cytotypes with 42, 44 and 38 chromosomes. We suggest a different set of events: first a pericentric inversion and a fusion/fission differentiated the races with 42 and 44 chromosomes; second a translocation and a tandem fusion from a karyotype with 2n = 42 would explain the variation observed in races with 38 chromosomes. Thus, we agree with Hoffmann et al. (2008 ) that the ancestral chromosomal race on the deepest branch could have 42 chromosomes, and that the hybrid zone arose later.
Intergeneric relationships among Vampyressina
Several studies, based mainly on morphological data, support Vampyressa as a monophyletic group (Goodwin 1963 , Owen 1987 , Wetterer et al. 2000 ). However, there is consensus from molecular data regarding its Vampyriscus and Chiroderma, indicating that V. bidens, V. brocki and V. nymphaea can be grouped instead in the genus Vampyriscus and that only V. pusilla, V. melissa, and V. thyone belong to the genus Vampyressa (Baker et al. 2000 , 2003 , Porter and Baker 2004 , Hoofer and Baker 2006 ).
Our results agree with the phylogenetic analysis of nuclear TSHB-I2 sequences made by Hoofer et al. (2008 ), strongly supporting the monophyly of Vampyriscus bidens with V. brocki (and their relationship with Chiroderma), the sister taxon relationship between Mesophylla and Vampyressa, as well as the polyphyly of Vampyressa. As pointed out by those authors, these results are in strong agreement with previous molecular studies by Baker et al. (2003 ).
Despite our results showing similar pairwise intergeneric relationships as the molecular studies, we found different branching between these groups. Although our analysis recovered a basal branching for the non-Stenodermatinae outgroups (PHA and CBR), and the monophyly of Stenodermatinae, the position of AOB within the Vampyressina disagrees with molecular and morphological data. Nevertheless, the presented topology is strongly supported by chromosomal data, namely the rearrangements shown in Fig. 2. For instance, both branches show an Y/15 translocation, but the resulting chromosomes have different morphologies, suggesting that they have different origins. Alternatively, they could have undergone further rearrangements since their divergence from their most recent common ancestor or be a case of hemiplasy (Robison et al. 2008 ) where the ancestral population could have contained both forms in polymorphic fashion, each cytotype becoming fixed in a different branch. This fusion is an easily recurrent rearrangement, since all the species in both branches have an X/15 fusion. During meiosis the Y and the free autosome (the other 15) will be very close because of pairing with the X/15, thus increasing the possibility that any chromatin break in the Y and free 15 would result in a fusion. Also, Artibeus obscurus has the X/15 fusion only, but not Y/15. This genus is divided into two groups, large and small Artibeus. For some authors the large should be organized in the subgenus Artibeus and small in Dermanura (Redondo et al. 2008 ; Wilson & Reader, 2005 ) while others consider that these groups are distinct genera (Baker et al. 2003 ; Solari et al. 2009 ). All karyotypes already described for large Artibeus have a XY1Y2 system, but there is variation in Dermanura: toltecus and aztecus have a XY1Y2 system (Baker, 1973 ) while watsoni and phaeotis have neo-XY (Baker, 1967 ; Hsu et al. 1968 ). A. cinereus has a XY1Y2 system in Central America (Baker, 1973 ) and neo-XY in South America (Souza & Araujo, 1990 ; Noronha et al. 2010 ). The neo-XY in Dermanura represents a third fusion between the Y and 15.
We tested many data matrices, using both PAUP and TNT, and all the resulting trees have the same branching pattern, suggesting that Vampyressina could be a polyphyletic subtribe resulting from convergent evolution, in a similar fashion to that observed in the subfamilies of nectarivorous bats (Ribeiro et al. 2003 ; Sotero-Caio et al. 2013 ). Although some branches have low bootstrap values, the decay index values give them a good support. Whereas the bootstrap is a more quantitative test, the Bremer index is qualitative, as is chromosomal variation.
Sex chromosome evolution in the subtribe Vampyressina
Translocations between an autosome and a sex chromosome are rarely fixed in speciation because of meiotic problems, including inactivation of the homologues fused to the X chromosome (Mattei et al. 1982 ). Phyllostomidae is an exception to this rule where there are subfamilies with most or all species having multiple or compound sex chromosome systems (e.g., Stenodermatinae and Carolliinae - Hsu et al. 1968 , Tuker 1986 ). In this work, we present for the first time a hypothesis explaining the sex chromosome evolution in the Vampyressina and describe the events that gave rise to the sex chromosome systems observed in M. macconnelli and V. thyone.
Our comparative analysis of all genera of vampyressine using a cytogenomic approach confirmed the molecular phylogeny previously described for this group, being independent evidence supporting that phylogeny. Our results also confirm that all genera of this subtribe have compound sex chromosome systems that originated from an X-autosome translocation, an ancestral condition observed in the Stenodermatinae subfamily. In Mesophylla we found a sex chromosome system never described before in mammals. We were able to trace all the chromosomal sex system evolution in vampyressine and show that it mirrors the phylogeny of this group. This work presents additional data supporting Hoofer & Baker (2006 ) hypothesis based on molecular studies regarding the polyphyly of the genus Vampyressa and its sister relationship to Mesophylla.
2n, diploid number; Ag-NOR, silver nitrate staining; AOB, Artibeus obscurus; CBR, Carollia brevicauda; CH, constitutive heterochromatin; CVI, Chiroderma villosum; DAPI, 4’, 6-diamidino-2-phenylindole; DEC, Diphylla ecaudata; DRO, Desmodus rotundus; DOP-PCR, Degenerate oligonucleotide-primed-polymerase chain reaction; DYO, Diaemus youngi; FISH, Fluorescence In Situ Hybridization; FN, Fundamental Number; MP, Maximum parsimony ; NOR, Nucleolar organizer region; PHA, Phyllostomus hastatus; PIN, Platyrrhinus incarum; MMA, Mesophylla macconnelli; PAUP, Phylogenetic analysis using parsimony; SSC, Saline sodium citrate; TBR, tree bisection reconnection; TNT, Tree analysis using new technology; VBI, Vampyriscus bidens; VBR, Vampyriscus brocki; VCA, Vampyrodes caraccioli; VTH, Vampyressa thyone.
We thank Manoel Rodrigues (Juris Ambientes Consultores), Flávio Eduardo Pimenta (Aotus Consultoria) for helping us in the field expedition to the area of Alcoa’s Juruti Bauxite mine. We thank Biodinamica-Rio, Aotus Consultoria, Eloiza Soarez, Fabio Sarmento, Fabio Augusto and Ramon Araujo for helping us with the expedition from Oriximina (Pará state) to Cariri (Amazonas state), Cibele Gomes de Sotero-Caio for the critical review of the manuscript. This study is part of the Doctoral thesis of AJBG who was a recipient of a CAPES (Conselho Aperfeiçoamento de Pessoal de Nível Superior) Scholarship in Genetics and Molecular Biology, UFPA.
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Fundação Amazônia Paraense (FAPESPA) on projects coordinated by CYN (Edital Universal, TO 064/2008; Edital BIONORTE-CNPq, Proc 552032/2010-7; Edital BIONORTE-FAPESPA, ICAAF 007/2011); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), on a Edital Pró-Amazônia project coordinated by CYN; the FAPESPA (Edital Vale – ICAAF 064/2011) and Banco Nacional de Desenvolvimento Economico e Social – BNDES (Operação 2.318.697.0001) on a project coordinated by JCP; CYN (308428/2013-7) and JCP (308401/2013-1) are granted as CNPq researchers.
Availability of data and materials
All the data supporting your findings are contained within the manuscript.
AJBG collected the samples, collaborated in all of the cytogenetic data and phylogenetic analyze, undertook the bibliographic review. CYN collaborated in all cytogenetic procedures and analyzes. LRRR collected part of the samples, collaborated with cytogenetic data and reviewed the paper. TCMB helped conceive the study and participated in developing the laboratory techniques. TFAR participated in the cytogenetic and phylogenetic analyses, and reviewed the manuscript. PCMOB and FY helped on writing the paper and discussed the data. MAFS helped on the development of the probes, participated in the cytogenetic analyses, and reviewed the manuscript. JCP helped conceive the study and participated in developing the laboratory techniques, and coordinated the writing of this paper. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent to publish
Ethics approval and consent to participate
The necessary euthanasia occurred in accordance with animal welfare guidelines established by the Animal Ethics Committee (Comitê de Ética Animal) from Universidade Federal do Pará (Permit 68–2015). JCP has a permanent field permit, number 13248 from “Instituto Chico Mendes de Conservação da Biodiversidade”. The Cytogenetics Laboratory from UFPa has a special permit number 19/2003 from the Ministry of Environment for samples transport and 52/2003 for using the samples for research.
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