Fig. 2

Phenotypic characterization of tomato flowers and fruits from wild type (a, c) and transgenic (b, d) #A plants, and analysis of the GBM5 gene expression in sepals (e) and fruits (f) of both wild type and 35S:GBM5 plants. Post-anthesis wild type (a) and transgenic #A (b) flowers look similar. Marked differences can be observed only in sepals accompanying the ripe fruit. Those of the wild type fruit (c) are green and have a leaf-like appearance while the corresponding sepals from the 35S:GBM5 ripe fruit (d) appear thick, fused at the proximal extremity and with a yellowish-orange color. Panels E (sepals) and F (fruits) show the relative expression of the GBM5 gene whose transcripts are found only in the samples obtained from the three different 35S:GBM5 plants (#A, #B, #D) studied in this work. G, mature green fruits and their relative sepals; B, breaker fruits and their relative sepals; R, red fruits and their relative sepals. Values (means of normalized expression) from real-time PCR analyses. Bars: standard deviations