Fig. 5From: Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterfliesGenomic in situ hybridization (GISH) in pachytene oocytes of Leptidea juvernica (a–d), L. sinapis (e–h) and L. reali (i–l). Female-derived genomic probes were labelled with fluorescein-12-dUTP (green) and chromosomes were counterstained with DAPI (blue). Figures (a–d), (e–h) and (i–l) show detailed analyses of sex chromosome multivalents W1-nZ1-n: (a, e, i) merged images of female genomic probes and DAPI staining; (b, f, j) DAPI images; arrows indicate DAPI-positive W-chromosome segments and heterochromatic blocks at the end of the W chromosomes; (c, g, k) hybridization pattern of the female genomic probes; the asterisk indicates an undifferentiated segment of one of the W chromosomes; (d, h, l) schematic drawings of the sex chromosome multivalents. Scale bar = 10 μmBack to article page