| Primer names | Sequence (5' - 3') | Reference | Target gene and group | Thermal profile1(°C) | Amplicon size (size of analyzed fragment2) |
---|---|---|---|---|---|---|
1 | gdhF | ATGGACAAACCAGCNAGYTT | [2] | gdh gene of S. oralis, S. mitis and S. infantis | 94°C,30s | ca. 659 bp |
55°C,30s | ||||||
(619 bp) | ||||||
gdhR | GCTTGAGGTCCCATRCTNCC | 72°C,60s | ||||
2 | MKR-F | TCCCGGTCAGCAAACTCCAGCC | [35] | gtfR of S. oralis | 94°C,30s | ca. 374 bp |
66°C,30s | ||||||
(330 bp) | ||||||
MKR-Rv | GCAACCTTTGGATTTGCAAC | 72°C,60s | ||||
3 | FN1164F16S | CGATGAGTAGGAGGAAGG | This study | 16S-23S rRNA-ITS-gene of F. nucleatum and F. periodonticum | 94°C,30s | ca. 637 bp |
52°C,30s | ||||||
72°C,60s | ||||||
(252 bp, incl. gaps) | ||||||
FN121R23S | CATTCGGAAATCCTAGAYTCTT |