Amphioxus encodes the largest known family of green fluorescent proteins, which have diversified into distinct functional classes

Table 2 Fluorescence and absorbance properties of amphioxus GFPs representative of five clades.

GFP	Abs. Max (nm)	FWHM (nm)	Ex. Max (nm)	FWHM (nm)	Em. Max (nm)	FWHM (nm)	Extinction Coefficient (M^-1 cm^-1) at 470 nm	Extinction Coefficient (M^-1 cm^-1) at 500 nm	Relative Fluorescence Intensity
GFPa1	497	45	500	38	516	39	48,100	120,900	100%
GFPe1	492	51	n.d.	n.d.	524	56	56,000	130,700	0.31%
GFPf1	504	60	n.d.	n.d.	n.f.	n.a.	13,400	25,500	n.a.
GFPd2	470	68	n.d.	n.d.	495	57	60,600	6,100	0.35%
GFPc1	493	55	n.d.	n.d.	521	56	63,900	98,800	0.33%

Excitation maximum was determined for emission set at 516 nm. Emission maximum was determined upon excitation at 500 nm for GFPa1 and at 465 nm for GFPe1, GFPf1, GFPd2, and GFPc1. Relative fluorescence intensity values are shown relative to the most fluorescent GFP, GFPa1. Abbrev.:Abs., absorbance, Ex., Excitation, Em., Emission, FWHM; full width half max, n.f., non-fluorescent; n.a., not applicable.

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