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Figure 4 | BMC Evolutionary Biology

Figure 4

From: ITS2 secondary structure improves phylogeny estimation in a radiation of blue butterflies of the subgenus Agrodiaetus (Lepidoptera: Lycaenidae: Polyommatus)

Figure 4

Conserved ITS2 secondary structure of the Polyommatina. The proximal stem of hybridized 5.8S (blue) and 28S (red) rDNA is included. Helices are numbered in Roman numerals. Two small helices are found near the beginning, which are referred to as helices I.a and I.b. The first (basal) internal bulge of helix II with two nucleotides mismatching one nucleotide is the typical U-U mismatch found in the second helix of ITS2 structures throughout the Eukaryota. Degree of conservation is displayed in colour grades from green (conserved) to red (unconserved). The complete structure represents the 51% consensus of aligned structures without gaps.

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