Phylogenetic reconstruction of the Nme protein family in chordates. The phylogenetic tree was constructed from a single multiple alignment. Bootstrap values for neighbor joining, maximum parsimony, and maximum likelihood methods, respectively, are indicated for each node. * indicates that the node does not exist in the corresponding tree. The consensus tree was calculated with the FIGENIX  automated phylogenomic annotation pipeline. Nme1-Nme2 subtree was removed from the main tree and studied separately (see Figure 5) for tetrapods and teleosts because of high sequence similarity and different evolutionary history. For each sequence, NCBI or Ensembl accession number and species are shown.