- Research article
- Open Access
Pleistocene glacial refugia across the Appalachian Mountains and coastal plain in the millipede genus Narceus: Evidence from population genetic, phylogeographic, and paleoclimatic data
© Walker et al; licensee BioMed Central Ltd. 2009
- Received: 11 June 2008
- Accepted: 30 January 2009
- Published: 30 January 2009
Species that are widespread throughout historically glaciated and currently non-glaciated areas provide excellent opportunities to investigate the role of Pleistocene climatic change on the distribution of North American biodiversity. Many studies indicate that northern animal populations exhibit low levels of genetic diversity over geographically widespread areas whereas southern populations exhibit relatively high levels. Recently, paleoclimatic data have been combined with niche-based distribution modeling to locate possible refugia during the Last Glacial Maximum. Using phylogeographic, population, and paleoclimatic data, we show that the distribution and mitochondrial data for the millipede genus Narceus are consistent with classical examples of Pleistocene refugia and subsequent post-glacial population expansion seen in other organismal groups.
The phylogeographic structure of Narceus reveals a complex evolutionary history with signatures of multiple refugia in southeastern North America followed by two major northern expansions. Evidence for refugial populations were found in the southern Appalachian Mountains and in the coastal plain. The northern expansions appear to have radiated from two separate refugia, one from the Gulf Coastal Plain area and the other from the mid-Atlantic coastal region. Distributional models of Narceus during the Last Glacial Maximum show a dramatic reduction from the current distribution, with suitable ecological zones concentrated along the Gulf and Atlantic coastal plain. We found a strong correlation between these zones of ecological suitability inferred from our paleo-model with levels of genetic diversity derived from phylogenetic and population estimates of genetic structuring.
The signature of climatic change, during and after the Pleistocene, on the distribution of the millipede genus Narceus is evident in the genetic data presented. Niche-based historical distribution modeling strengthens the conclusions drawn from the genetic data and proves useful in identifying probable refugia. Such interdisciplinary biogeographic studies provide a comprehensive approach to understanding these processes that generate and maintain biodiversity as well as the framework necessary to explore questions regarding evolutionary diversification of taxa.
- Coastal Plain
- Glacial Maximum
- Mismatch Distribution
- Spotted Salamander
- Paleoclimatic Data
The profound effect of Pleistocene glaciation upon species distributions in the Northern Hemisphere has been well documented [1–7]. As continental ice sheets advanced into temperate areas, rendering once suitable areas uninhabitable, many northern taxa experienced extreme range reduction and fragmentation into refugia, while other populations and taxa were decimated entirely. Subsequently, as the ice sheets receded, recolonization of these historically glaciated areas by northernmost populations in refugia occurred rapidly. The genetic population structure in these organisms often reflects geological patterns [1, 2, 7]. Rapid, post-glacial expansions distribute internal (ancestral) haplotypes throughout large, previously glaciated, geographic areas. Furthermore, exponential growth of founding populations and subsequent resource competition may prevent other refugial populations from expanding simultaneously . Taxa currently occupying previously glaciated and historically unsuitable areas are often characterized by low levels of genetic diversity [1–3, 5–7].
Approximately 22,000 to 19,000 years before present, global temperatures were significantly lower than today and the volume of land-based ice was at its maximum . Recently, niche-based species' distribution modeling has been applied to construct habitat suitability models for taxa during the Last Glacial Maximum [9–11]. Increasing availability of high-resolution temporal and spatial paleoclimatic data have greatly aided and improved fine-scale modeling of species' historical geographic movements. The number of studies showing the presence of genetic signatures indicative of glacial refugia among terrestrial and aquatic species has increased dramatically since the 1990's [4, 10]. These models provide a relatively novel approach to exploring historical distributions of species, as they often coincide with previously hypothesized refugial regions .
With the exception of one morphologically distinct species in Florida, populations of Narceus appear phenotypically homogeneous throughout their range. However, their rather simplified morphology and current nomenclature belie a number of taxonomic complexities that have historically plagued this geographically widespread genus. At present, the genus comprises three species (N. americanus, N. annularis, and N. gordanus), which contain no fewer than 16 synonyms. Although resolving the taxonomy of this group is not our primary focus, the complex history of the genus (discussed herein) has likely contributed to confusing species boundaries within this ubiquitous and interesting group of millipedes. Given the history (evolution and nomenclatural) of the genus, as suggested by Shelley , molecular data are likely needed to begin to properly disentangle the species limits within Narceus. The union of phylogeographic and population genetic principles provides the toolkit necessary to investigate the historical distribution of these lineages and, ultimately, helps disclose the mechanisms that maintain current levels of diversity and population structuring.
Here we present the first phylogeographic study of the genus Narceus. We show that the genetic structure and current distribution of the genus Narceus (east of the Mississippi) reflects a complex history of refugia in the Appalachian Mountains and coastal plain of North America. We examine our data for patterns that should be consistent with a Pleistocene refugia hypothesis. First, there should be a profile of phylogeographic and genetic diversity that distinguishes coastal plain versus all other populations. Secondly, populations currently occupying historically unsuitable habitat should show genetic signatures of rapid range expansion. And last, high probability of habitat suitability from paleo-models of historical distributions should be coincident with those populations having relatively higher levels of genetic diversity.
Summary of data and phylogenetic analyses
Mitochondrial sequence data (1354 bp) were obtained for individuals (n = 269) from 96 localities; 427 positions were parsimony informative (GenBank accession numbers FJ213618-FJ213764). The average ingroup sequence divergence was 7.3% with a range of < 0.01% – 14.6%. Most localities are represented by > 3 individuals. However at 10 localities we were only able to obtain a single individual. One hundred and fifty-three unique haplotypes were recovered following alignment. Twelve of these haplotypes were found at multiple localities, one of which occurred at 12 widely distributed northern localities. Of the 86 localities surveyed where more than a single individual was sampled (90%), 21 contained a single haplotype.
For the Bayesian analysis, GTR + I + Γ model of molecular evolution was inferred for the 16S region and HKY + I for both the tRNA-Val and 12S partitions. Of 8 million generations, 800,000 were discarded as burnin, resulting in a tree with a mean ln-likelihood value of -9768.09.
Niche-based distribution modeling
Genetic divergence and diversity computed for each of the major Narceus clades; clade names correspond to those designated in Figure 2.
Mean pairwise distance
π Tajima, 1996
θ Tajima, 1996
Florida panhandle/southern Appalachian
When we optimize the character "coastal plain" onto the haplotype phylogeny and then compare it to the optimization of suitable habitat during the Pleistocene (derived from the 0.2 threshold value in the MAXENT output) we find a very high level of similarity (Figure 7); we only recovered a single haplotype with a high probability of paleo-model habitat suitability not located in the coastal plain. A large majority of the coastal plain haplotype lineages occupy areas that would have been suitable refugia. Furthermore, each clade with a coastal plain interior node has a non-coastal plain sister clade. The Mississippi/Louisiana clade is sister to the exclusively non-coastal, shallowly divergent Northwestern clade; likewise, the Florida panhandle/Southern Appalachian clade is sister to the Southeastern clade, which comprises a non-coastal sister to a subclade showing a radiation from and back to the coastal plain. Genetic diversity for the coastal clade consisting of only two haplotypes (NCCR02II and NCCR02I) could not be calculated due to limited sampling. However, we do see a pattern similar to other sister clades within the tree.
Using an integrative approach that employs genetic and geospatial data, this study provides a straightforward example of Pleistocene refugia and post-glacial colonization. The star-like patterns and widely distributed haplotypes in our phylogeographic analyses taken together with significantly negative Fu's Fs values, support two major rapid expansions from coastal refugia into northern, historically glaciated areas. The southern populations of Narceus are characterized by relatively high levels of genetic diversity, isolation by distance, multimodal mismatch distributions, and an absence of widely distributed ancestral haplotypes. Similar genetic patterns across geographic regions attributed to Pleistocene related phenomena have been detected in numerous animals including salamanders [5, 6], frogs , voles , mice , bears , and grasshoppers . Our niche-based paleodistribution model also provides compelling evidence for southern refugia, specifically relegated to the coastal plain.
Pleistocene refugia and post-glacial expansion
Glacial pulses during the Pleistocene profoundly affected animal distributions in North America, and species in the genus Narceus are no exception. As continental glaciers advanced, the destruction of habitat necessitated the displacement or extinction of northern taxa. Populations of displaced northern taxa suffered severe bottlenecks and hence decreased genetic diversity, while southern taxa that persisted during glacial advances were able to maintain high levels of diversity . An example of this phenomenon was provided by Church et al.  in eastern tiger salamanders. They showed a distinct split between eastern and western populations. Within the eastern lineage, two possible refugia were identified: the Blue Ridge Mountains and the coastal plain of the Carolinas. They hypothesized that the populations located in mountain refugia have remained isolated after deglaciation whereas the coastal plain refugia were the source for northern post-glacial expansion along the east coast.
We found a similar pattern in Narceus. The basal-most clade in the phylogeny (Southern Appalachian) occurs south of any previously glaciated areas in the southern Appalachians, with populations from the Blue Ridge Mountains of Virginia to the southern extent of Appalachia in eastern Alabama. This clade also exhibits isolation by distance and relatively high levels of genetic diversity with no unique haplotypes occupying more than a single locality. The Texas/Louisiana clade, the next basal-most clade, also demonstrates the genetic signature of a refugial past. Within the Texas/Louisiana clade, mismatch distributions (Figure 5) indicate highly fragmented populations , with each locality sampled having at least one unique haplotype – another common characteristic of refugia populations [24, 25]. With our sampling scheme, we found a single distributional extension into northern Indiana (Figures 2 &4), at first believed to be an anthropogenic introduction. However, we did detect isolation by distance within this clade. Without further sampling, the phylogenetic placement of this population remains enigmatic. A similar pattern of radiation was detected between the Mississippi/Louisiana clade and its sister, the Northwestern clade (Figures 2 &4).
The Mississippi/Louisiana clade exhibits isolation by distance, relatively high levels of genetic diversity, and a lack of shared haplotypes among localities – all characteristics consistent with a deep refugial history [4, 6]. Its sister, the Northwestern clade, is widely distributed west of the Appalachians (Figure 4). This clade exhibits relatively low levels of genetic divergence and is distributed across a large geographic distance. It also contains a single haplotype (ILOJ514II) found in eight localities in four states (Illinois, West Virginia, Virginia, and Tennessee). The haplotype network indicates six other haplotypes connected to ILOJ514II by = 2 mutational steps, resulting in a star-like pattern (Figure 3). A pattern also observed for rapidly expanded populations of spotted salamanders , wood frogs , and other taxa [17, 21, 26–29]. The star-like pattern and the mismatch plot of pairwise differences in the Northwestern clade (Figure 5) are both consistent with relatively rapid expansion, as might be expected for a quick founding colonization into a newly deglaciated suitable area. Indeed, the results of the paleodistribution models (Figure 6) show that the majority of habitat currently occupied by the Northwestern clade was likely unsuitable 21,000 years ago. This pattern reflects a northern radiation from the Gulf coast northward and west of the Appalachians, resembling that of the Texas/Louisiana clade.
Similar to patterns found in eastern tiger salamanders , we detected a radiation from the mid-Atlantic coastal plain north through upstate New York; however, this radiation appears to have extended west as well, like that seen in the spotted salamander (Figure 4) . The Northern clade has three widely distributed haplotypes all of which show star-like TCS networks (Figure 3). Fu's Fs, an indicator of rapid range expansion [30, 31], was significant and negative for the Northern clade. Despite multiple lines of evidence indicating rapid range expansion, isolation by distance was detected for this clade. Although long-distance dispersal can also account for patterns similar to those seen for this clade, millipedes are non-vagile and thus unlikely to disperse, via a single event, across a long distance. Therefore, the phylogeographic pattern is more likely the result of rapid range expansion.
The Southeastern and Florida panhandle/southern Appalachian clades represent separate refugial populations that have remained isolated. The Southeastern clade indicates a rather complex phylogeographic pattern in which mismatch plots and isolation by distance analyses were consistent with those of refugial populations. However, Fu's Fs was significant and negative in the Southeastern clade, suggesting a rapid expansion. This clade is widespread throughout the southeast, and character map reconstructions suggest multiple radiations to and from the coastal plain within this clade. These pulses may have been fairly recent, a scenario consistent with significant negative values for Fs obtained here. The Florida panhandle/southern Appalachian area represents a coastal refugium with an east-to-west phylogeographic break at the Apalachicola/Chattahoochee/Flint (ACF) River Basin, a well documented geographic barrier to gene flow for multiple taxa . There is a single radiation of these millipedes forming a monophyletic group extending southeast of the ACF barrier into the southern Appalachians. The Fall Line (the interface between the coastal plain and the Appalachian foothills) does not seem to prevent radiations of these millipedes; the area immediately east and running north-to-south of the ACF basin may serve as a riparian corridor from the coastal plain to the southern Appalachians .
High-resolution models of past distributions for species provide a promising new tool in the field of phylogeography [9, 10]. A recent study by Waltari et al.  addressed concordance between Pleistocene refugia predicted using traditional biogeographic methods and niche-based paleodistribution modeling. Fourteen of the twenty North American terrestrial taxa tested showed significant spatial correlations between traditional and niche-based modeling methods for identifying Pleistocene refugia . Agreement between these methods suggests that paleodistribution models may well provide useful guidelines for biogeographic studies.
Our Last Glacial Maximum model for Narceus (Figure 6b) indicates a major reduction in habitat suitability that stands in stark contrast to the present-day model. Hypotheses of refugia along the Gulf and Atlantic coastal plains are consistent with indicated areas of highest predicted suitability in the niche-based distribution models. The character map of haplotypes by region, illustrates a disproportionate number of haplotypes from these areas on the phylogeny (Figure 7). A correlation between genetic diversity and habitat suitability from our Pleistocene model suggests that the model's highest predicted probabilities, in fact, do show higher levels of genetic diversity and are consistent with those patterns expected from refugial areas. These refugia hypotheses are consistent with all mismatch plots and isolation by distance tests for clades occurring in coastal areas.
Somewhat inconsistent with the refugia hypotheses was the low habitat suitability modeled in the southern Appalachian and Texas coastal plain regions of our paleodistribution model. Although these areas show very low probability (0.007% – 1.390%) of having suitable habitat during the Last Glacial Maximum (Figure 6), it is apparent that millipede populations were able to survive there. During the Last Glacial Maximum, suitable habitat in these areas may have been reduced into numerous microrefugia within the overall southern Appalachian refugium that could lead to the diversity currently found in these clades. Under this hypothesis, overall habitat suitability would appear to be very low; however, populations may have persisted within suitable microrefugia.
As discussed in the Background section, Narceus presently comprises three nominal species, two of which appear to be morphologically homogenous. Narceus gordanus is the only species that appears to be morphologically distinct and has a rather limited distribution, endemic to peninsular Florida and extending north to South Carolina . Individuals assigned to N. gordanus are represented in the phylogeny (Figure 2) by a clade nested squarely within populations that are morphologically indistinguishable and clades that likely represent the remaining two species. This phylogenetic placement renders N. americanus and N. annularis (sensu lato) paraphyletic. Moreover, as has been demonstrated for other millipede groups [e.g., Jamaican Anadenobolus; [35, 36]], cryptic species may be a problem for species delineation within this genus. Further work will be needed to resolve these issues, both in terms of careful morphological analyses and intensive collecting west of the Mississippi. An integrative approach that takes into account biogeographic patterns, analyses of morphology, phylogenetic history, and climatic envelope will likely provide the framework necessary to investigate species boundaries in this widespread, morphologically homogeneous genus [see ].
The signature of climatic change on the distribution of the millipede genus Narceus is evident in the mtDNA data presented here. Niche-based historical distribution modeling strengthens the conclusions drawn from the genetic data and proves useful in identifying areas of probable Pleistocene refugia. This study provides the first molecular phylogeographic and ecological modeling data for the millipede genus Narceus. The methods used here provide an integrative biogeographic approach to understand processes generating patterns of biodiversity, and an approach to explore the evolutionary diversification of species, populations, and organismal distributions.
Molecular Protocols and sequence alignment
Live millipedes collected in the field were transported to the laboratory, where legs or heads were removed and stored in RNA later (Qiagen, Valencia, CA) at -80°C. Voucher specimens were stored in 80% ethanol and will be eventually deposited in the Field Museum of Natural History collection (Chicago, IL). Genomic DNA was extracted from leg or head tissue using a Qiagen DNeasy Tissue Kit (Qiagen, Valencia, CA) and a mitochondrial region spanning 12S, tRNA-Val, and 16S genes was amplified via a standard polymerase chain reaction (PCR) with primers LR-J-12887dip (CCG GTC TGA AAC TCA GAT CAT GT) and SR-N-145xxdip1 (GTA TAT CGC TGT CGT CAG A). Products were column purified and sequenced with an ABI Hitachi 3130 Genetic Analyzer and/or an ABI PRISM 377 automated DNA sequencer. Raw sequence data were edited with Sequencher 4.1. Multiple sequence alignment was performed with MUSCLE . Aligned data were partitioned by comparison with a published mitochondrial genome of Narceus .
One hundred and fifty three unique haplotypes (1354 bp) were used as terminal taxa for phylogenetic analyses. The molecular data were divided into 3 partitions: 16S, tRNA-Valine, and 12S. Models of nucleotide evolution for each partition were selected using MRMODELTEST 2.2  following the Akaike Information Criterion (AIC). Phylogenetic reconstructions were created using both maximum likelihood in GARLI 0.95 (1000 bootstrap replicates; program available at http://www.bio.utexas.edu/faculty/antisense/garli/Garli.html) and Bayesian inference in MRBAYES 3.1.2 . With MRBAYES, two simultaneous analyses were run, each with eight chains sampling every 100 generations. Convergence was assumed as the average standard deviation of split frequencies stabilized below 0.01. TRACER 1.4 (available at http://tree.bio.ed.ac.uk/software/tracer) was employed to examine parameter values, and a majority-rule consensus tree was produced after the removal of trees saved prior to burn-in. Statistical parsimony, as implemented by TCS , was used as an unbiased criterion for assigning subclades.
Isolation by distance was tested within each major mtDNA lineage using a Mantel test of correlation between genetic and geographic distance matrices implemented in the software package IBD . Using DNASP , mismatch distributions of pairwise differences within each major mtDNA lineage were produced and plotted against the expected distributions under a null model of exponential population growth to explore the possibility of recent range expansions . Unimodal distributions are expected for clades with recent population expansions, whereas multimodal distributions are expected for clades with more phylogeographic structure. In terms of refugia scenarios, an unimodal distribution of pairwise differences would be expected for shallowly divergent lineages that currently occupy previously glaciated areas, and multimodal distributions would be expected for deeply divergent lineages that currently occupy areas once suitable for glacial refugia . We employed DNASP to compute Fu's Fs (1000 replicates), shown in a comparative study by  to be one of the best indicators of rapid population growth.
Phylogenetic patterns for coastal plain populations were investigated by scoring haplotypes as coastal or "montane" and then using the parsimony character state reconstruction in MESQUITE  to optimize these two character states onto the haplotype phylogeny. As a direct confirmation to assess whether coastal plain regions may have served as refugia for Narceus during the glacial maximum, values were extracted from the niche-based distribution model using the paleoclimatic data set (see methods below). We scored two character states: 0) area suitable during the Pleistocene (P ≥ 0.20); and 1) area unsuitable during the Pleistocene (P < 0.20).
Environmental variables used to produce niche-based distribution models.
Annual mean temperature
Temperature seasonality (standard deviation *100)
Mean temperature of wettest quarter
Mean temperature of driest quarter
Mean temperature of warmest quarter
Mean temperature of coldest quarter
Precipitation seasonality (Coefficient of variation)
Precipitation of wettest quarter
Precipitation of driest quarter
We tested for significant correlations (Spearman's rho) between the Pleistocene habitat suitability and genetic diversity (mean pairwise difference; π; θ)  among major mtDNA lineages. Given that we have produced an accurate model and the fact the Narceus have limited dispersal capabilities, we would expect to see higher levels of genetic diversity in lineages currently occupying areas of high habitat suitability in our Pleistocene distribution model. Because MAXENT output is continuous, a threshold must be selected in order to create a binary distribution model where all pixels are either predicted as present or absent (or suitable/unsuitable). We chose a conservative threshold based on the lowest predicted value associated with any of the observed sampling sites for the current model [Pearson et. al.'s 'LPT'; ]. This approach maintains a zero omission error (i.e., zero false negatives), thereby maintaining that each pixel predicted "present" has conditions at least as suitable as those where the species is known to occur . This threshold was then applied to the paleodistribution model and used as the basis of a character matrix. Thus, each haplotype in the inferred Bayesian phylogeny was marked as being located in either suitable or unsuitable habitat during the Last Glacial Maximum. Character maps were then reconstructed using parsimony in MESQUITE  to infer species movement and/or stasis between the past and present. Haplotypes located in areas predicted as suitable during the Last Glacial Maximum outline possible refugia areas, while those in locations predicted as unsuitable might be indicative of post-glacial range expansions.
This project was supported by National Science Foundation grants DEB-0529715 and CNS 0619285. We thank David Beamer and Brent Hendrixson for their assistance in collecting specimens. Drs. Petra Sierwald, Trip Lamb, and Bill Shear provided helpful insight and support during all phases of this study. The final version of this manuscript benefited greatly from the insight provided through two anonymous reviews.
- Avise JC: Molecular Population-Structure and the Biogeographic History of a Regional Fauna – a Case-History with Lessons for Conservation Biology. Oikos. 1992, 63 (1): 62-76. 10.2307/3545516.View ArticleGoogle Scholar
- Bernatchez L, Wilson CC: Comparative phylogeography of nearctic and palearctic fishes. Molecular Ecology. 1998, 7 (4): 431-452. 10.1046/j.1365-294x.1998.00319.x.View ArticleGoogle Scholar
- Taberlet P, Fumagalli L, Wust-Saucy AG, Cosson JF: Comparative phylogeography and postglacial colonization routes in Europe. Molecular Ecology. 1998, 7 (4): 453-464. 10.1046/j.1365-294x.1998.00289.x.View ArticlePubMedGoogle Scholar
- Hewitt G: The genetic legacy of the Quaternary ice ages. Nature. 2000, 405 (6789): 907-913. 10.1038/35016000.View ArticlePubMedGoogle Scholar
- Church SA, Kraus JM, Mitchell JC, Church DR, Taylor DR: Evidence for multiple pleistocene refugia in the postglacial expansion of the eastern tiger salamander, Ambystoma tigrinum tigrinum. Evolution. 2003, 57 (2): 372-383.View ArticlePubMedGoogle Scholar
- Zamudio KR, Savage WK: Historical isolation, range expansion, and secondary contact of two highly divergent mitochondrial lineages in spotted salamanders (Ambystoma maculatum). Evolution. 2003, 57 (7): 1631-1652.View ArticlePubMedGoogle Scholar
- Wilson AB: Genetic signature of recent glaciation on populations of a near-shore marine fish species (Syngnathus leptorhynchus). Molecular Ecology. 2006, 15 (7): 1857-1871. 10.1111/j.1365-294X.2006.02911.x.View ArticlePubMedGoogle Scholar
- Yokoyama Y, Lambeck K, De Deckker P, Johnston P, Fifield LK: Timing of the Last Glacial Maximum from observed sea-level minima. Nature. 2000, 406 (6797): 713-716. 10.1038/35021035.View ArticlePubMedGoogle Scholar
- Richards CL, Carstens BC, Knowles LL: Distribution modelling and statistical phylogeography: an integrative framework for generating and testing alternative biogeographical hypotheses. Journal of Biogeography. 2007, 34 (11): 1833-1845. 10.1111/j.1365-2699.2007.01814.x.View ArticleGoogle Scholar
- Waltari E, Hijmans RJ, Peterson AT, Nyári AS, Perkins SL, Guralnick RP: Locating Pleistocene Refugia: Comparing Phylogeographic and Ecological Niche Model Predictions. PLoS ONE. 2007, 2 (6): e563-10.1371/journal.pone.0000563.PubMed CentralView ArticlePubMedGoogle Scholar
- Yesson C, Culham A: A phyloclimatic study of Cyclamen. BMC Evolutionary Biology. 2006, 6 (1): 72-10.1186/1471-2148-6-72.PubMed CentralView ArticlePubMedGoogle Scholar
- Rafinesque CS: Annals of nature, or annual synopsis of new genera and species of animals and plants discovered in North America. First Annual Number for. 1820, 1: 1-20.Google Scholar
- Shelley RM, McAllister CT, Medrano MF: Distribution of the milliped genus Narceus Rafinesque, 18210 (Spirobolida : Spirobolidae): Occurrences in New England and west of the Mississippi River; A summary of peripheral localities; and first records from Connecticut, Delaware, Maine, and Minnesota. Western North American Naturalist. 2006, 66 (3): 374-389. 10.3398/1527-0904(2006)66[374:DOTMGN]2.0.CO;2.View ArticleGoogle Scholar
- Chamberlin RV: On Some Genera and Species of American Millipeds. Bulletin of the University of Utah. 1943, 34 (6): 5-7.Google Scholar
- Clement M, Posada D, Crandall KA: TCS: a computer program to estimate gene genealogies. Molecular Ecology. 2000, 9 (10): 1657-1659. 10.1046/j.1365-294x.2000.01020.x.View ArticlePubMedGoogle Scholar
- Mardulyn P: Phylogeography of the Vosges mountains populations of Gonioctena pallida (Coleoptera : Chrysomelidae): a nested clade analysis of mitochondrial DNA haplotypes. Molecular Ecology. 2001, 10 (7): 1751-1763. 10.1046/j.0962-1083.2001.01307.x.View ArticlePubMedGoogle Scholar
- Joy DA, Feng XR, Mu JB, Furuya T, Chotivanich K, Krettli AU, Ho M, Wang A, White NJ, Suh E, Beerli P, Su X: Early origin and recent expansion of Plasmodium falciparum. Science. 2003, 300 (5617): 318-321. 10.1126/science.1081449.View ArticlePubMedGoogle Scholar
- Tajima F: The amount of DNA polymorphism maintained in a finite population when the neutral mutation rate varies among sites. Genetics. 1996, 143 (3): 1457-1465.PubMed CentralPubMedGoogle Scholar
- Lee-Yaw JA, Irwin JT, Green DM: Postglacial range expansion from northern refugia by the wood frog, Rana sylvatica. Molecular Ecology. 2008, 17: 867-884.View ArticlePubMedGoogle Scholar
- Conroy CJ, Cook JA: Phylogeography of a post-glacialcolonizer: Microtus longicaudus (Rodentia : Muridae). Molecular Ecology. 2000, 9 (2): 165-175. 10.1046/j.1365-294x.2000.00846.x.View ArticlePubMedGoogle Scholar
- Rajabi-Maham H, Orth A, Bonhomme F: Phylogeography and postglacial expansion of Mus musculus domesticus inferred from mitochondrial DNA coalescent, from Iran to Europe. Molecular Ecology. 2008, 17 (2): 627-641.View ArticlePubMedGoogle Scholar
- Cooper SJB, Ibrahim KM, Hewitt GM: Postglacial Expansion and Genome Subdivision in the European Grasshopper Chorthippus parallelus. Molecular Ecology. 1995, 4 (1): 49-60. 10.1111/j.1365-294X.1995.tb00191.x.View ArticlePubMedGoogle Scholar
- Slatkin M, Hudson RR: Pairwise Comparisons of Mitochondrial-DNA Sequences in Stable and Exponentially Growing Populations. Genetics. 1991, 129 (2): 555-562.PubMed CentralPubMedGoogle Scholar
- Vogler AP, Desalle R: Phylogeographic Patterns in Coastal North-American Tiger Beetles (Cicindela-Dorsalis Say) Inferred from Mitochondrial-DNA Sequences. Evolution. 1993, 47 (4): 1192-1202. 10.2307/2409985.View ArticleGoogle Scholar
- Walker BH: Biodiversity and Ecological Redundancy. Conservation Biology. 1992, 6 (1): 18-23. 10.1046/j.1523-1739.1992.610018.x.View ArticleGoogle Scholar
- Huang S, He SP, Peng ZG, Zhao K, Zhao EM: Molecular phylogeography of endangered sharp-snouted pitviper (Deinagkistrodon acutus; Reptilia, Viperidae) in Mainland China. Molecular Phylogenetics and Evolution. 2007, 44 (3): 942-952. 10.1016/j.ympev.2007.05.019.View ArticlePubMedGoogle Scholar
- Rambau RV, Robinson TJ, Stanyon R: Molecular genetics of Rhabdomys pumilio subspecies boundaries: mtDNA phylogeography and karyotypic analysis by fluorescence in situ hybridization. Molecular Phylogenetics and Evolution. 2003, 28 (3): 564-575. 10.1016/S1055-7903(03)00058-7.View ArticlePubMedGoogle Scholar
- Schneider C, Moritz C: Rainforest refugia and evolution in Australia's Wet Tropics. Proceedings of the Royal Society of London Series B-Biological Sciences. 1999, 266 (1415): 191-196. 10.1098/rspb.1999.0621.View ArticleGoogle Scholar
- Zaccara S, Stefani F, Crosa G: Diversity of mitochondrial DNA of the endangered white-clawed crayfish (Austropotamobius italicus) in the Po River catchment. Freshwater Biology. 2005, 50 (7): 1262-1272. 10.1111/j.1365-2427.2005.01385.x.View ArticleGoogle Scholar
- Ramos-Onsins SE, Rozas J: Statistical properties of new neutrality tests against population growth. Mol Biol Evol. 2002, 19 (12): 2092-2100.View ArticlePubMedGoogle Scholar
- Fu YX: Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics. 1997, 147 (2): 915-925.PubMed CentralPubMedGoogle Scholar
- Pauly GB, Piskurek O, Shaffer HB: Phylogeographic concordance in the southeastern United States: the flatwoods salamander, Ambystoma cingulatum, as a test case. Molecular Ecology. 2007, 16 (2): 415-429. 10.1111/j.1365-294X.2006.03149.x.View ArticlePubMedGoogle Scholar
- Naiman RJ, Decamps H, Pollock M: The Role of Riparian Corridors in Maintaining Regional Biodiversity. Ecological Applications. 1993, 3 (2): 209-212. 10.2307/1941822.View ArticleGoogle Scholar
- Keeton WT: A taxonomic study of the milliped family Spirobolidae (Diplopoda: Spirobolida). Memoirs of the American Entomological Society. 1960, 17: 1-146.Google Scholar
- Bond JE, Sierwald P: Cryptic speciation in the Anadenobolus excisus millipede species complex on the Island of Jamaica. Evolution. 2002, 56 (6): 1123-1135.View ArticlePubMedGoogle Scholar
- Bond JE, Sierwald P: Molecular taxonomy of the Anadenobolus excisus (Diplopoda : Spirobolida : Rhinocricidae) species-group on the Caribbean island of Jamaica. Invertebrate Systematics. 2003, 17 (4): 515-528. 10.1071/IS03004.View ArticleGoogle Scholar
- Bond JE, Stockman AK: An integrative method for delimiting cohesion species: finding the population-species interface in a group of californian trapdoor spiders with extreme genetic divergence and geographic structuring. Systematic Biology. 2008, 57 (4): 628-646. 10.1080/10635150802302443.View ArticlePubMedGoogle Scholar
- Edgar RC: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research. 2004, 32 (5): 1792-1797. 10.1093/nar/gkh340.PubMed CentralView ArticlePubMedGoogle Scholar
- Lavrov DV, Boore JL, Brown WM: Complete mtDNA sequences of two millipedes suggest a new model for mitochondrial gene rearrangements: Duplication and nonrandom loss. Mol Biol Evol. 2002, 19 (2): 163-169.View ArticlePubMedGoogle Scholar
- Nylander JAA: MrModeltest v2. Program distributed by author. 2004, Evolutionary Biology Centre, Uppsala University, v2.2Google Scholar
- Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003, 19 (12): 1572-1574. 10.1093/bioinformatics/btg180.View ArticlePubMedGoogle Scholar
- Bohonak AJ: IBD (isolation by distance): A program for analyses of isolation by distance. Journal of Heredity. 2002, 93 (2): 153-154. 10.1093/jhered/93.2.153.View ArticlePubMedGoogle Scholar
- Rozas J, Sanchez-DelBarrio JC, Messeguer X, Rozas R: DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics. 2003, 19 (18): 2496-2497. 10.1093/bioinformatics/btg359.View ArticlePubMedGoogle Scholar
- Maddison WP, Maddison DR: Mesquite: a modular system for evolutionary analysis. Version 2.01. 2007, [http://mesquiteproject.org]Google Scholar
- Phillips SJ, Anderson RP, Schapire RE: Maximum entropy modeling of species geographic distributions. Ecological Modelling. 2006, 190 (3–4): 231-259. 10.1016/j.ecolmodel.2005.03.026.View ArticleGoogle Scholar
- Guisan A, Zimmermann NE: Predictive habitat distribution models in ecology. Ecological Modelling. 2000, 135: 147-186. 10.1016/S0304-3800(00)00354-9.View ArticleGoogle Scholar
- Roura-Pascual N, Suarez A, Gómez C, Pons P, Touyama Y, Wild AL, Peterson AT: Geographic potential of Argentine ants (Linepithema humile Mayr) in the face of global climate change. Proceedings of the Royal Society of London B. 2005, 271 (1557): 2527-2535. 10.1098/rspb.2004.2898.View ArticleGoogle Scholar
- McGuire JA, Linkem CW, Koo MS, Hutchison DW, Lappin AK, Orange DI, Lemos-Espinal J, Riddle BR, Jaeger JR: Mitochondrial introgression and incomplete lineage sorting through space and time: Phylogenetics of crotaphytid lizards. Evolution. 2007, 61 (12): 2879-2897. 10.1111/j.1558-5646.2007.00239.x.View ArticlePubMedGoogle Scholar
- Elith J, Graham CH, Anderson RP, Dudik M, Ferrier S, Guisan A, Hijmans RJ, Huettmann F, Leathwick JR, Lehmann A, Li J, Lohmann LG, Loisell BA, Manion G, Moritz C, Nakamura M, Nakazawa Y, Overton J, Peterson AT, Phillips SJ, Richardson K, Scachetti-Pereira R, Schapire E, Soberon J, Williams S, Wisz MS, Zimmerman NE: Novel methods improve prediction of species' distributions from occurrence data. Ecography. 2006, 29 (2): 129-151. 10.1111/j.2006.0906-7590.04596.x.View ArticleGoogle Scholar
- Pearson RG, Raxworthy CJ, Nakamura M, Peterson AT: Predicting species distributions from small numbers of occurrence records: a test case using cryptic geckos in Madagascar. Journal of Biogeography. 2006, 34 (1): 102-117. 10.1111/j.1365-2699.2006.01594.x.View ArticleGoogle Scholar
- Hijmans RJ, Cameron SE, Parra JL, Jones PG, Jarvis A: Very high resolution interpolated climate surfaces for global land areas. International Journal of Climatology. 2005, 25 (15): 1965-1978. 10.1002/joc.1276.View ArticleGoogle Scholar
- Graham CH, Ron SR, Santos JC, Schneider CJ, Moritz C: Integrating phylogenetics and environmental niche models to explore speciation mechanisms in dendrobatid frogs. Evolution. 2004, 58 (8): 1781-1793.View ArticlePubMedGoogle Scholar
- Kutzbach JE, Guetter PJ: The Influence of Changing Orbital Parameters and Surface Boundary-Conditions on Climate Simulations for the Past 18000 Years. Journal of the Atmospheric Sciences. 1986, 43 (16): 1726-1759. 10.1175/1520-0469(1986)043<1726:TIOCOP>2.0.CO;2.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.