Table 1 Sequences of primers used for PCR amplification and sequencing.
Primer name | Sequence (5' -- 3') | Comment |
|---|---|---|
PPE38F | TTTTCGGTGTGGATTGTCT | 3398 bp amplicon for H37Ra-like genotype, 1331 bp amplicon for RvD7 genotype. |
PPE38R | GCCAGGGATTTCCAACGAC | Â |
PPE38IntF | ATGTCGGCGGAGTTGGGTAAG | 1351 bp amplicon for H37Ra-like genotype, no product for RvD7 genotype. |
PPE38IntR | TAGCCTGACCAGCCGACAACT | Â |
21delF | GGGGATGATGCCGATGC | 111 bp amplicon for wild-type genotype, 90 bp amplicon for 21del genotype. |
21delR | ACACTGGGCCGAGCCTG | Â |
IS5' | GGTACCTCCTCGATGAACCAC | IS6110-binding sequencing primer used to determine region upstream of IS6110. |
Xho1 | TTCAACCATCGCCGCCTCTAC | IS6110-binding sequencing primer used to determine region downstream of IS6110. |
plcA5' | CAAATGTCCGGGACAAGG | Primes from the 5' region of plcA. Used to PCR and sequence the region between plcA and PPE38 in conjunction with the PPE38IntF primer in M. canettii isolates. |