Figure 3
From: Evolutionary history of selenocysteine incorporation from the perspective of SECIS binding proteins
The SBP SID and RBD are highly conserved across Eukarya. (A) An extraction of the SBP2 and SBP2L SID region from the global multiple sequence alignment described for Figure 2 prior to GBlocks treatment. The non-conserved region between 464-505 (human SBP2 numbering) was deleted. (B) An extraction of the SBP2 and SBP2L RBD region as in (A). Nonconserved sequences from M. brevicollis, E. huxleyi, and T. gondii that introduced large gaps in the region were deleted. In addition the sequence from S. purpuratus was omitted from this alignment due the presence of several large non-conserved regions that disrupted the entire global alignment. Positions that are consistently variable between SBP2 and SBP2L are indicated with red arrows and the identity/number refers to the position in human SBP2. Underlined sequence corresponds to the GBlocks output that was used to generate the tree in Figure 2. Blue shaded boxes indicate conserved motifs that are only sporadically found in unicellular organisms.