Deviations from Hardy-Weinberg expectations. Deviation from Hardy-Weinberg expectations (HWE) by position in the Ambystoma linkage map  and by study pond: (A) Bluestone, (B) Melindy, (C) Pond H, (D) Sycamore, (E) Toro. Positive FIS indicates heterozygote deficit and negative FIS indicates heterozygote excess. The red point is GNAT1. Vertical dashed lines separate linkage groups, which are plotted end to end from largest to smallest .