Evolutionary relationships of Hypocreaceae SLPs based on neighbor joining analysis. The phylogram is rooted using the Bacillus subtilis subtilisin Carlsberg protein (accession P00780) as an outgroup. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. SLP families and subfamilies are indicated by black or grey square brackets respectively. E. festucae sequences are marked by black circles. Note: FOXG_14793 does not group with sf3 (the vacuolar SLPs) or sf4 (characterized by FGSG_09382) but falls into a group by itself. Additional analysis showed it groups with proteases from P. anserina and N. fischeri.