Table 2 Primers used for cloning and sequencing
Primer name | Sequence1 |
|---|---|
CTL06263'F | GCTCCAGACTTCCAACTC |
CTL06295'R | TGTAAAGCCATGAAGAAGG |
5CT373X | CGGCTCGAG ATGCCTTACGGAACTCG |
3CT374H | GCAAGCTTACAAATGGATTTTATTAGC |
5CT373N | GGTCAT ATGCCTTACGGAACTCG |
3CT373B | GCTGGATCCTTATTGGATAACAGCAGGC |
5CT373X128W | CAGAATACGTAGAGTTTTTCCCAACTTGGATCGATGATGAAATCGCAGAAT |
3CT373X128W | ATTCTGCGATTTCATCATCGATCCAAGTTGGGAAAAACTCTACGTATTCTG |
5CT372X | GGGCTCGAG ATGTCCTTCCGTTCGGTTTTAC |
3CT374R | CGCGAATTCTTACAAATGGATTTTATTAGC |
5CT373R115G | GGAGAGCTCATCGGGGGCTGGGCAG |
3CT373R115G | CTGCCCAGCCCCCGATGAGCTCTCC |
pBAD-Fwd2 | CGTCACACTTTGCTATGC |
pBAD-Rev | GATTTAATCTGTATCAGGCTG |
CTseq1 | GATCAGCTACATGATCGGAG |
CTseq2 | TCAGTACTCACTCTATGCAATAG |
CTseq3 | CCGTGTTTATAGCGTGCGAGC |
CTseq4 | GTGATCAGCTCCATGATCG |
T7-Promoter | GTAATACGACTCACTATAGGG |
T7-Term | GCTAGTTATTGCTCAGCGG |