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Table 3 List of PCR primers designed to assay fungi for glycoside hydrolase family 32 (GH32) gene presence.

From: Friend or foe? Evolutionary history of glycoside hydrolase family 32 genes encoding for sucrolytic activity in fungi and its implications for plant-fungal symbioses

Primer

Primer sequence 5'→3'

Direction

Length

Tm°C

INV1-1F

TTYATGAAYGAYCCNAAYGG

Forward

20

53.96

INV1-FA1F

CARCAYTGGGGNCAYGCNAC

Forward

20

62.76

INV1-B1F

GGNAAYCARCAYTGGGGNCAYGC

Forward

23

67.97

INV2-1F

AACTGGATSAAYGAYCCNAAYGG

Forward

23

59.6

INV4-2F

TTYTTYCARCAYAAYCCNAC

Forward

20

50.74

INV5A-1F

GGITGGHTIAAYGAYCCNTGYG

Forward

22

61.88

INV5B-A1F

GGICRIATYGGNGAYCCNTG

Forward

20

58.39

INV5B-B1F

GGITGGATGAAYGAYCCNATG

Forward

21

60.57

INV5B-B1.1F

GGITGGATGAAYGAYCC

Forward

17

55.65

INV5C-1F

GGITGGMTGAAYGAYCCTTG

Forward

20

61.74

INV6-1F

TGYCCIGARTGYYCNGA

Forward

17

52.34

INV1-1R

ACYTTIGGRTCNCKRAA

Reverse

17

46.05

INV1-B1R

TTNARNGTRTARATNGCNACIACICC

Reverse

26

55.76

INV-1-B2R

GGRAARAANCCNGAIGTRTT

Reverse

18

52.11

INV1-2R

TAITICCARTTNGANGCCCA

Reverse

20

62.52

INV1-2.1R

TAITICCARTTRTTNGCCCA

Reverse

20

61.54

INV2-2R

GGICCICCIGGRTTNAGNCC

Reverse

20

61.29

INV4-1R

TCIGGIACYTCCCANCC

Reverse

17

55.45

INV5A-1R

RCAICCIGTRAANACNCC

Reverse

18

49.03

INV5B-1.1R

CRTAIGGRTCNCGRAANGC

Reverse

19

54.69

INV5B-1.3R

CRTAIGGRTCNCGRAANCC

Reverse

19

53.52

INV5B-1.4R

CRAAIGGRTCYCTRAANCC

Reverse

19

50.09

INV5B-1.5R

CRAAIGGRTCNCGRAANCC

Reverse

19

53.52

INV5C-1R

GTGAYGTTCCANCCYTCNGGNGG

Reverse

23

68.6

INV6-2R

CAIGAYTTIGCNGCRTACCA

Reverse

20

61.22

  1. Primer pair combinations and PCR conditions that correspond to a particular GH32 sequence are given in Additional file 3. Tm, melting temperature.