Experimental evolution and genome sequencing reveal variation in levels of clonal interference in large populations of bacteriophage φX174

Table 4 Frequency of Mutations in Four Genomic Regions.

Mutation site	Protein	Putative function	Control, Benign^a	Harsh^a
181	C, K (DNA maturation and accessory proteins)	Burst size	2	0
563, 576, 587, 593, 788	D, E (Lysis protein and its regulatory region)	Lysis timing	10	0
1033, 1295, 1302, 1611, 2772	F, G (Capsid proteins)	Attachment, stability, assembly	7	0
2971, 2973, 3039, 3129	H (Pilot protein)	Eclipse rate: DNA ejection into host	0	5

^a Numbers are the frequency that mutations from a particular functional category were observed in independent evolution lines under the indicated treatments.
Only adaptive mutations (i.e., satisfying criteria in Table 3) from endpoint populations are shown.

ISSN: 2730-7182