The evolutionary costs of immunological maintenance and deployment

Table 5 Results from contrast repeated measures ANOVA for changes in fecundity after injection.

Yeast-Limited Diet
	Full Model		Tests of Each Contrast Separately
	d.f.	Roy's Max Root (approx. F)	d.f.	V3 vs. V4	V3 vs. V5	V3 vs. V6	V3 vs. V7
Hemiclone Line	39,343	3.122***	39,343	1.910**	2.375***	1.601*	1.693**
Injection	4,341	7.343***	2,343	5.638**	0.210	0.094	1.429
HK vs. Sterile Needle	4,340	1.362	1,343	0.051	0.122	0.001	2.807
HK vs. Uninjected	4,340	6.498***	1,343	9.041**	0.418	0.126	0.423
St. Needle vs. Uninj .	4,340	4.952***	1,343	7.811**	0.090	0.153	1.058
Hemiclone × Injection	78,343	1.337*	78,343	0.778	0.628	0.844	0.798
BLOCK	4,342	103.303***	3,343	14.430***	15.919 ***	11.041 ***	79.833 ***
Injector	4,343	16.612***	4,343	5.509***	4.006**	12.507***	7.039***
Number of Females	4,340	4.286 ***	1,343	6.203*	10.384**	15.008***	10.365**
Yeast-Unlimited Diet
Hemiclone Line	39, 340	2.875***	39,340	1.727**	1.941**	0.653	1.615*
Injection	4,338	2.868*	2,340	4.882**	2.025	2.526	2.611
HK vs. Sterile Needle	4,337	2.466*	1,340	6.348*	3.284	5.045*	5.205*
HK vs. Uninjected	4,337	2.078	1,340	8.080**	2.591	1.052	1.505
St. Needle vs. Uninj .	4,337	0.629	1,340	0.110	0.048	1.435	1.068
Hemiclone × Injection	78,340	1.340*	78,340	0.685	0.834	1.159	1.034
BLOCK	4,339	72.312***	3,340	7.417***	4.992**	12.418***	43.740***
Injector	4,340	17.893***	4,340	11.535***	12.615***	3.556**	10.770***
Number of Females	4,337	37.617***	1,340	3.020	6.711*	100.065***	88.784***

The response matrix (M-Matrix) for the full model contrasts fecundity in Vial 3 (pre-injection fecundity) with each subsequent vial. Multivariate test statistics for the full model are based on approximate F-tests generated using Roy's Max Root.
* p < 0.05, ** p < 0.01, *** p < 0.001.

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