Phylogenetic trees of the depressant toxin family of Boi and BmK scorpions. (A) Full transcript, (B) leader-coding domain and (C) mature-coding domain of depressant toxins from both Boi and BmK scorpions were aligned by the MUSCLE algorithm and edited manually with Jalview. Trees were constructed by the neighbor-joining method, based on maximum likelihood composite. Numbers on the branches are bootstrap percentages, while brackets mark the genus origin of the toxins. Boi transcripts are shadowed in blue.