Analysis of the coding potential of VMCH-p8 and its variants in human and macaque tissues. (A) Schematic representation of PMCHL ORF1, encoding the putative VMCH-p8 protein, present on unspliced RNA, and its variants, ORF1a and ORF1b, present on spliced RNAs. (B) Sequence comparison of PMCHL1 and PMCHL2 ORF1, ORF1a and ORF1b. The ORF1 epitope is underlined. The putative nuclear localisation signal (NLS) is shaded in grey. The variable C-terminal parts are boxed. (C) Sequence comparisons of PMCHL1 ORF1, ORF1a and ORF1b in Homo sapiens (HSA), Pan troglodytes (PTR), Pongo pygmaeus (PPY) and Macaca fascicularis (MFA). The variable C-terminal parts are boxed. (D) Specificity of the anti-VMCH-p8 antiserum. In Western blots, immune anti-VMCH-p8 serum detects the recombinant GST-VMCH-p8 protein (12.5 ng) migrating at 34 kD (arrow). (E) Western blot analysis of the expression of VMCH-p8 and its variants in vivo in human and macaque tissues. GST-VMCH-p8 (12.5 ng), human adult testis proteins (25 μg), new-born (NB) and fetal (F) hippocampus (HPC) and prefrontal cortex proteins (CX) (80 μg each), and macaque supplementary motor area (SMA), cerebellum (CB), prefrontal cortex (CX) and visual area (VA) proteins (80 μg each) were analysed using the anti-VMCH-p8 antiserum. The arrow points to GST-VMCH-p8 migrating at 34 kD.