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Figure 4 | BMC Evolutionary Biology

Figure 4

From: Module evolution and substrate specificity of fungal nonribosomal peptide synthetases involved in siderophore biosynthesis

Figure 4

Schematic representation of phylogenetic relationships among A and among C domains within each lineage. A domain relationships for each lineage and between lineages are color coded as in Fig. 3 and Additional files 3 and 6. C domain relationships are indicated by arrows for each lineage. The NPS2 lineage relationships are indicated in the top half of figure and the NPS1/SidC lineage relationships in the bottom half of figure. Scheme is based on phylogenetic analyses of A (Fig. 3, see Additional files 3, 4 and 6and C domains. Spsib1, ChNPS2, FgNPS2, FgNPS1, AnSidC, Umfer3, and Umsid2 are representative of architectural Types I-V (Fig. 2). Also mapped on the A domains are predicted substrates adenylated by each domain, based on structural modeling (Table 2, Fig. 7). SER = serine, GLY = glycine, ALA = alanine, AHO = N5-acyl-N5-hydroxy-L-ornithine. Within the NPS2 lineage, ChNPS2 and FgNPS2 C domain analyses clearly indicate that C2 domains are related, as are C3 domains. Thus the difference in protein architecture in this region is presence/absence of an A domain between C2 and C3. A similar argument can be made for the difference in protein structure between C1 and C2 C domains of Spsib1 vs those of ChNPS2 and FgNPS2. For the NPS1/SidC lineage, A and C domain analyses of FgNPS1, AnSidC, and Umfer3 clearly indicate that there is a one to one relationship for all A and all C domains. Examination of Umsid2, however, indicates that Umsid2 module 1 A domain is related to the module 2 A domains of the other members of this group, while Umsid2 modules 2 and 3 A domains are related to the C-terminal module of the other members of this group. Umsid2 appears to lack the N-terminal A domain of other NPS1/SidC members, since the C domain from module 1 is related to the C domains of module 2 of the rest of the lineage. Similarly the C domains from Umsid2 module 2, 3, 4 are related to the C domains of modules 3, 4, 5 of the rest of the NPS1/SidC lineage.

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