Figure 3From: Module evolution and substrate specificity of fungal nonribosomal peptide synthetases involved in siderophore biosynthesisMaximum likelihood tree of all AMP domains examined in this study demonstrating two separate lineages of ferrichrome synthase NRPSs. N-terminal A domains of both lineages group together and C-terminal domains of both lineages group together (thick vertical bars). NPS2, module 2 groups with the C-terminal modules, while NPS1/SidC module 2 and Dothideomycete NPS2 module D.3 group with the N-terminal modules. Numbered nodes indicate duplications inferred from the reconciliation analysis. White circles indicate a duplication inferred due to incongruence of the gene tree with the species tree (see Additional files 2 and 6), while red circles indicate a duplication inferred due to the presence of two copies of a gene in the same species. Bootstrap support values greater than 50% are reported above branches. Note that the A domains of SidE module 1 group as directly sister to all ferrichrome synthetase A domains examined here, while A domains of SidE module 2 group with A domains of other types of C. heterostrophus NRPSs. For species and protein Accession numbers see Additional file 1. Nomenclature: e.g., Ch_ NPS2_AAX09984_AMP3_4 indicates C. heterostrophus, protein accession number AAX09984, AMP module 3 of a total of 4 (see Fig. 2). For Bayesian analysis, see Additional files 3 and 6.Back to article page