Primers | Â |
---|---|
Fw primer (5'to 3') | GCCTCAAACCCTTTCACCT |
Rv primer (5'to 3') | CGCTTACGGGTCCTCTGAT |
Reagents | Â |
MasterMix | 10 ul, 2× |
Primer mix | 150 uM final each |
H2O | 4.1 ul |
cDNA | 5 ul, ~5 ng |
Conditions | Â |
 | 50°C 10 min |
 | 95°C 15 min |
39 × | 95°C 30 sec |
 | 60°C 20 sec |
 | 72°C 40 sec |
 | 78°C 1 sec |
 | Plate read |
 | 72°C 1 min |
Melting curve | 55 – 90°C, 0.5°C, 1 sec |
 | 72°C 5 min |
 | 15°C Hold |