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Figure 4 | BMC Evolutionary Biology

Figure 4

From: Evolutionary proteomics identifies amino acids essential for ligand-binding of the cytokinin receptor CHASE domain

Figure 4

Identification of amino acid residues of the CRE1/AHK4 CHASE domain important for ligand binding. (A) Effect of point mutations in the CRE1/AHK4 CHASE domain on the specific binding of trans-[2-3H]zeatin. For the localization of the mutated sites see Fig. 1B. H482Q is a control carrying a mutated histidine residue of the cytoplasmic domain. Data are means ± S.D. from measurements with two different E. coli clones for each construct. (B) Western blot with whole protein extracts of an aliquot of E. coli cells used for the binding assay shown in (A). For protein detection a mouse-anti-GST antibody (GST B-14, Santa Cruz Biotechnology) was used.

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