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Figure 4 | BMC Evolutionary Biology

Figure 4

From: The invariant phenylalanine of precursor proteins discloses the importance of Omp85 for protein translocation into cyanelles

Figure 4

Binding of pFNR to the precursor binding domains of Omp85-like proteins. Radioactively labeled pFNR (top), pFNR ΔAFV (middle) or pSSU (bottom; lane 1, 100% input) was incubated with an Ni-agarose [28] affinity matrix without coating (empty column, EC, lane 7–9) or coated with the N-terminal domain of Alr2269 (Ana69N, lanes 1–3) or pea Toc75 (psToc75N, lanes 4–6). The flow through (F; lanes 1, 4, 7), material from the last wash step (W; lanes 2, 5, 8) and the eluate (E; lanes 3, 6, 9) was subjected to SDS-PAGE and binding visualized by autoradiography.

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